Alocasia Tissue Culture: Sustainable Plant Health and Diversity

April 09, 2024

Over the years, science has advanced and used the best techniques to its benefit. One such technique is tissue culture, which helps propagate and multiply different types of plants to increase their growth in a controlled environment. One such plant on the list is the Alocasia plants.

Why is this method carried out for the Alocasia plants, and how does it benefit the human race? If you are searching for the same query, then lucky for you, you have landed on the right page. Hop on below to learn everything you should know about Alocasia Tissue Culture.

Why is it Important to Carry Out Tissue Cultures?

When you think of tissue culture, one can easily decipher that it refers to the multiplication of a plant type in a controlled environment. However, the question is why it is carried out and what makes it so important. Well, one of the significant reasons tissue culture is so influential is its effectiveness in helping many developing countries increase their food and crop production.

However, is this the only benefit that tissue cultures provide? No, there is so much more to it. Here are some of the other tissue culture advantages that might be relevant to you:

The new platelets of any plant can be grown within a short amount of time.

When grown in a controlled environment, the new platelets are bound to be free from viruses or diseases.

The cultivation process is not bounded by the season and can be carried out throughout the year.

 You only require a small amount of the tissue plant to carry out the alocasia tissue culture.

Alocasia sanderiana 'Nobilis'

A Step-By-Step Guide to Alocasia Tissue Culture

Now that you know the benefits tissue culture can provide, we are sure you must be intrigued to learn a little about the methods through which you can carry out the culture for Alocasia plants, right? Well, if that is the case, then here is a multiple study verified ways for alocasia tissue culture:

1. Plant Material and Seed Preparation

Collect the fruits from the alocasia plants and then wash them. Once done, rinse them with sterile distilled water to remove dust. Now, carefully separate the plants' seeds by hand and let them dry at room temperature for two weeks.

2. Seed Viability Test

Absorb some (100 or more) seeds of the Alocasia plants in sterile distilled water at 25 +/- 2 for about 18 hours. This will help sift the seeds' teguments and activate the plant's enzyme systems. Then, immerse the seeds in 1% tetrazolium solution and incubate them in a dark area for 6 hours at 45 degrees.

When the incubation period is over, wash the seeds with sterile water and observe them under a microscope for color changes. Calculate the viability percentage by dividing the number of stained embryos by the total number of embryos multiplied by 100.

3. Seed Surface Sterilization

Wash the dried seeds first under regular tap water for 30 minutes for seed surface sterilization. Then, surface sterilize the seeds with 40% Clorox and a few drops of tween-20. Shake the seeds at 80 rpm in an orbital container for 20 minutes.

4. Pre-Germination Treatment

This step will help break the seed dormancy and maximize seed germination. All you have to do is follow this procedure to treat seeds:

First, scarify the seeds in 30% sulphuric acid for around 15 minutes and then rinse them with distilled water for another 10 minutes to remove the traces of acid solution. Now, surface sterilize them again with 40% Clorox for 20 minutes and re-wash them with distilled water three times for one minute each.

Now, culture the seeds in an MS medium with 30 grams of L-1 sucrose, and 2.78 grams of L-1 Gelrite without any plant growth regulator. Adjust the pH to 5.7, add agar and ten autoclaves at 121 degrees Celsius for 20 minutes, and incubate the culture at 25 degrees with 16 hours of photoperiod through cool white fluorescent lights.

5. In Vitro Shoot Induction and Multiplication

For the in vitro shoot induction, use the four-week-old grown in-vitro seeds. Take out the cotyledon, hypocotyl, and the root carefully. Then, inoculate 2-3 cm shoot tip into the MS medium supplemented with 3 mg L-1 cytokinin 6-benzyl amino purine.

6. For Callus Induction

Wash the seeds with 30% sulphuric acid for 15 minutes and carry out surface sterilization with 40% Clorox for 20 minutes. Now, wash the explants with sterile distilled water to remove the traces of Clorox. Keep the disinfected seeds in a jar with a 25 ml medium for callus induction. Incubate the culture at 25 degrees under cool fluorescent lights.

7. For In Vitro Rooting

From different shoot cultures, culture the isolated shoots (2-3 cm) into glass jard with 20 ml of MS medium, which is supplemented with indole-3-acetic acid at 0.5 mg L-1, 30g L-1 sucrose, and 8 grams L-1 agar under 16/8 h light/dark photoperiod.

8. For Acclimatization

After four weeks, please remove the well-grown rooted platelets from the vessels and wash them with tap water. Then, rinse the roots with water to remove the agar medium.

Keep the plantlets from the alocasia tissue culture in a room with a temperature of 25 degrees Celsius 5 before transferring it to the greenhouse. After five days, plant the platelets into small plastic pots in a soil medium with a combination of peat moss and topsoil at a 2:1 ratio.

Alocasia odora varigated 'Batik'
Alocasia longiloba 'Pink Dragon'
Alocasia frydek
Alocasia reginula 'Black Velvet'

What Benefits Does Alocasia Tissue Culture Provide?

The alocasia plants are a great pick when carrying out the tissue culture process. This is because the growth of alocasia tissue culture, in the long run, can provide multiple incredible benefits from its plants. Here are a few of these benefits:

1. Air Purification

The large green leaves of alocasia plants help filter the air. They trap particulates from the environment and release oxygen in return. This helps purify the environmental air, ultimately benefiting human health.

2. Prebiotics

Alocasias contain prebiotics, which help promote the growth of the gut bacteria and hence aid in a healthy digestive system. The leaves of the alocasia plant pass through the digestive system and work as a 'food' for probiotics and good bacteria in the gut microbiome.

3. Congestion Relief

The folk medicine practices also use alocesia to treat chest congestion and other respiratory discomfort. Though not many scientific studies have been done on this, early research indicates that the minerals, vitamins, and other antioxidants present in the Alocasia plant may help cleat the airways and promote healthy lung function.

4. Prevents and Treats Fungal Infections

The extracts exhibit antifungal properties in the labs when tested on a few species of fungi, which help infect plants, animals, and humans. Many researchers even claim alocasia plants to be highly therapeutic against fungal infections.

5. Potential Treatment for Skin Condition

Skin conditions are the worst for some people, but do you know that alocasia plants may be beneficial for treating many skin conditions like eczema, acne, and allergic rashes?

6. Anti-Inflammatory Effects

The alocasia plants have compounds that contain anti-inflammatory properties. The extracts tested during the alocasia tissue cultures also showed the inhibition of specific pro-inflammatory messaging molecules in the body. Alocasia plants may aid in certain health conditions by reducing inflammation in tissues.

Where to Find the Best Alocasia Plants?

Now that you know all the fantastic benefits of Alocasia plants, you're sure you must be looking for a place with these exceptional plants, right?

The Foshan Youngplants is a tissue culture manufacturing and selling business that provides its customers with high-quality and reasonably priced disease-free plug plants. The company has a significant customer base established on reliability and excellence courtesy of its excellent services and high-quality sales.

The company uses state-of-the-art technology and practices that produce healthy and strong plants. While Alocasia is Foshan Youngplants' best-selling product, the company doesn't limit itself to this plant product only. Foshan Youngplants also offers its consumers a wide range of other tissue-culture plants like fruit trees, vegetable plants, ornamental plants, etc.


If you have heard about tissue culture and want to read more about it, we hope this article was helpful for you. Producing and planting Alocasia plants is one of the best scientific decisions ever made, and these plants vouch for their benefits. So, if you plan on purchasing the Alocasia plants to keep in their house or plant them in your garden, we suggest you definitely check out Foshan Youngplants. The company excels in its domain and will not leave you disappointed.


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