Ungakhula Kanjani Izithombo Ze-Colocasia Ezinempilo Evela Emasikweni Ezicubu

Amaqabunga aqinile futhi anempilo ezitshalo ze-Colocasia, ngokuvamile aziwa ngokuthi izindlebe zendlovu, angaba isengezo esimangalisayo kunoma iyiphi ingadi. Ukukhulisa izithombo ze-Colocasia ezinempilo kusuka kusiko lwezicubu kuyindlela ephumelelayo yokuqinisekisa ukukhula okufanayo kanye nezitshalo ezingenazifo. Ukusakazwa kwesiko lezicubu kuvumela ukukhiqizwa ngobuningi bezitshalo ezifanayo ezinezici ezifiselekayo endaweni elawulwayo. Kulo mhlahlandlela ophelele, sizocubungula izinyathelo ezihilelekile ekukhuliseni izithombo zeColocasia ezinempilo kusuka kusiko lwezicubu.

Ukuqonda Isiko Lwezicubu

Isiko lezicubu kuyindlela yokusabalalisa ebandakanya ukukhohliswa kwamaseli ezitshalo endaweni yaselabhorethri ukuze kukhiqizwe izitshalo ezintsha. Le ndlela ivame ukusetshenziselwa ukusabalalisa izitshalo ezinezici ezifiselekayo, njengokumelana nezifo noma ukukhula ngokushesha. Endabeni yezithombo zeColocasia, isiko lezicubu livumela ukuphindaphinda ngokushesha kwezitshalo ezinezici ezingaguquki. Inqubo iqala ngokukhethwa kwesitshalo somzali esinempilo esinezici ezifiselekayo, ezizosebenza njengomthombo wesiko lezicubu.

Ukuze kuqalwe isiko lezicubu, izingcezu ezincane zezicubu, ezifana neqabunga noma izingxenye zesiqu, zithathwa esitshalweni esingumzali futhi zifakwe inzalo ukuze kukhishwe noma yikuphi ukungcola. Lawa masampula ezicubu abe esefakwa endaweni enomsoco equkethe izakhamzimba ezibalulekile namahomoni okukhula ukuze akhuthaze ukukhula kwezitshalo ezintsha. Indawo elawulwayo yelabhorethri yesiko lezicubu iqinisekisa izimo ezifanele zokukhula kwezithombo ezinempilo ze-Colocasia.

Ukulungisa Indawo Yokukhula

Impumelelo yokusabalalisa isiko lezicubu incike ekubunjweni kwendawo yokukhula esetshenziselwa ukusekela ukukhula kwamaseli ezitshalo. Indawo yokukhula kufanele inikeze zonke izakhi ezidingekayo, amavithamini, namahomoni ukukhuthaza ukuthuthukiswa kwezithombo zeColocasia. Indawo yokukhula evamile yokusabalalisa isiko lezicubu zezitshalo ze-Colocasia iqukethe inhlanganisela yoshukela, amavithamini, amaminerali, nezilawuli zokukhula kwezitshalo.

Ukuze ulungise i-medium yokukhula, qala ngokuncibilikisa amakhemikhali adingekayo kanye nezakhamzimba emanzini a-distilled. I-pH yendawo ephakathi kufanele ilungiswe ibe yibanga elifanele lokukhula kwezitshalo, ngokuvamile cishe phakathi kuka-5.8 kuya ku-6.0. I-agar, uhlobo lwento ye-gelatinous, ivame ukungezwa endaweni yokukhula ukuze iqiniswe futhi inikeze ukusekela kwezitshalo ezikhulayo. Uma indawo yokukhula isilungisiwe, ikhishelwa ezitsheni ezingenalutho, njengezitsha ze-petri noma amashubhu okuhlola, alungele ukwethulwa kwamasampula ezicubu.

Ukuqala I-Tissue Culture

Isinyathelo esilandelayo ekukhuleni izithombo ze-Colocasia ezinempilo kusuka kusiko lwezicubu ukuqaliswa kwesiko lezicubu elabhorethri. Amasampula ezicubu ezithathwe esitshalweni esingumzali abekwe ngokucophelela phezu kwendawo yokukhula eyinyumba ezitsheni. Amasampula abekwe ngendlela yokuthi abe nokuxhumana okuqondile nendawo, okuvumela ukudluliswa kwezakhi kanye nama-hormone okukhula ukuze akhuthaze ukukhula kwawo.

Ngemuva kokuthi amasampula ezicubu ebekwe endaweni yokukhula, iziqukathi ziyavalwa futhi zibekwe endaweni elawulwayo enezinga lokushisa elilungile nezimo zokukhanyisa. Amaseli ezitshalo kumasampula ezicubu azoqala ukuhlukana futhi ahlukanise, akhe ama-plantlets amancane ngokuhamba kwesikhathi. Ukuqapha njalo isiko lezicubu kubalulekile ukuze kuqinisekiswe ukuthi izitshalo zikhula kahle futhi azinamagciwane.

I-Subculturing kanye Nokuphindaphindeka

Njengoba ama-plantlets ekhula kusiko lwezicubu, azodinga ukuguqulwa noma adluliselwe endaweni entsha yokukhula ukuze kuthuthukiswe ukukhula nokuphindaphindeka. I-Subculturing ihilela ukukhipha ama-plantlets esitsheni sokuqala bese uwabeka esitsheni esisha esinendawo yokukhula entsha. Le nqubo isiza ukuvimbela ukuminyana kwezitshalo futhi izinikeze indawo edingekayo kanye nezakhamzimba ukuze ziqhubeke nokukhula kwazo.

Ukuphindaphindeka kwama-plantlets kusiko lwezicubu kutholakala ngokuphindaphindwa kwe-subculturing yezitshalo ezikhulayo. Ngokudlulisela ama-plantlets endaweni entsha yokukhula ngezikhathi ezithile, inani lezitshalo ezikhiqizwa kusiko lwezicubu lingakhula kakhulu. Izindlela ezifanelekile zokutshala i-subculturing, njengokusetshenziswa kwamathuluzi angenalutho neziqukathi, zibalulekile ukuvimbela ukungcoliswa nokuqinisekisa impilo namandla ezithombo zaseColocasia.

Ukulinganisa Izithombo

Ngemuva kokuthi izithombo ze-Colocasia sezithuthukile izimpande futhi zizinzile kusiko lwezicubu, zilungele ukujwayela imvelo yangaphandle. I-Acclimatization yisinyathelo esibalulekile enqubweni yokusabalalisa isiko lezicubu, njengoba kuvumela izithombo ukuthi zivumelane nezimo zemvelo ngaphandle kwelabhorethri. Ukuchayeka kungazelelwe ekushintsheni kwezinga lokushisa, umswakama, nokukhanya kungase kucindezele izithombo futhi kubeke engozini ukukhula nokuphila kwazo.

Ukuze wenze izithombo ze-Colocasia zijwayelane, ziveze kancane kancane endaweni yangaphandle ngokuhamba kwesikhathi. Qala ngokunciphisa amazinga womswakamo azungeze izithombo futhi kancane kancane wandise ukuchayeka ekukhanyeni kwemvelo. Bazise emazingeni okushisa e-ambient futhi banikeze amanzi anele kanye nemisoco ukuze basekele ukukhula kwabo. Qaphela ngokucophelela izithombo ngesikhathi senqubo yokujwayela ukuze uqinisekise ukuthi zijwayela kahle endaweni yazo entsha.

Sengiphetha, ukukhulisa izithombo ze-Colocasia ezinempilo kusuka kusiko lwezicubu kuyinqubo evuzayo evumela ukukhiqizwa kwezitshalo ezifanayo nezingenazifo ezinezici ezifiselekayo. Ngokuqonda izimiso zokusabalalisa isiko lezicubu nokulandela izinyathelo ezidingekayo, abalimi bangasakaza ngempumelelo izithombo zeColocasia endaweni elawulwayo. Ngokunakekelwa okufanele nokunaka, lezi zithombo zingakwazi ukujwayela indawo yangaphandle futhi ziphumelele ezingadini, zengeze ubuhle nokuhlukahluka endaweni.