Isinyathelo Ngesinyathelo Umhlahlandlela Wokusakazwa Kwe-Alocasia Tissue Culture

Ukusabalalisa isiko lezicubu kuyindlela ebalulekile yokwandisa izitshalo ezifana ne-Alocasia, eyaziwa ngamahlamvu azo amangalisayo nokubukeka okuyingqayizivele. Ngokusebenzisana nesiko lezicubu zezitshalo, ungakwazi ukukhiqiza ngokushesha inani elikhulu lenzalo efana nezakhi zofuzo, ezingenalo izinambuzane nezifo. Kulo mhlahlandlela wesinyathelo ngesinyathelo, sizokuhamba ngenqubo yokusabalalisa isiko lezicubu ze-Alocasia, kusukela ekukhetheni isitshalo esifanele ukuya ekunakekeleni izitshalo zakho ezintsha.

Ukukhetha Okufanelekile Kwezitshalo

Ngaphambi kokuthi uqale inqubo yesiko lezicubu, kubalulekile ukukhetha isitshalo esifanele ongasebenza ngaso. Khetha izitshalo ezinempilo ezingenazo izinambuzane noma izifo. Khetha izitshalo ezinokukhula okuqinile, okusha, njengoba lokhu kuzokunika ithuba elihle kakhulu lokuphumelela. Kubalulekile futhi ukukhetha izitshalo eziyiqiniso ekuthayipheni, okusho ukuthi azikakahlanganiswa noma aziguqulwanga nganoma iyiphi indlela. Lokhu kuzoqinisekisa ukuthi izitshalo zakho ezintsha ziyefana ngokofuzo nesitshalo esingumzali.

Lapho ukhetha izinto zezitshalo zesiko lamathishu, kungcono ukhethe amahlumela amancane, akhula ngenkuthalo. Lawa mahlumela azoba namaseli e-meristematic amaningi, okungamaseli anesibopho sokukhula nokuthuthuka. Gwema ukukhetha iziqu noma amaqabunga amadala, njengoba lawa angase angabi nawo amangqamuzana e-meristematic amaningi futhi angase angavuseleli kalula. Uma usukhethe isitshalo esifanele, usulungele ukudlulela esinyathelweni esilandelayo senqubo yesiko lezicubu.

Ukwenziwa inzalo ebusweni

Esinye sezinyathelo ezibaluleke kakhulu ekusakazweni kwesiko lezicubu ukuvala inzalo ebusweni. Le nqubo ihilela ukususa noma yikuphi ukungcola ebusweni bezinto zesitshalo ukuze kuvinjwe ukuthi zikhule endaweni ephakathi futhi ezingase zilimaze izitshalo ezintsha. Ukuze ukhiphe amagciwane esitshalweni, uzodinga ukusebenzisa inhlanganisela yamakhemikhali, njenge-bleach noma i-ethanol, kanye nezindlela zomzimba, njengokuqhaqhazela noma ukuvuvuka.

Ukufaka inzalo esitshalweni, qala ngokuyigeza ngesisombululo samanzi kanye nesihlanzi ukuze ususe noma yikuphi ukungcola noma imfucumfucu. Okulandelayo, hlambulula impahla yesitshalo emanzini ukuze ususe okokuhlanza, bese uyidlulisela kusixazululo se-bleach noma i-ethanol ukuze inzalo. Ngemva kokufaka inzalo esitshalweni, yihlanza izikhathi eziningana emanzini angenalutho ukuze ususe noma imaphi amakhemikhali asele. Uma impahla yesitshalo isivalekile, ungadlulela esinyathelweni esilandelayo senqubo yesiko lezicubu.

Eqala Isiko

Ngemva kokufaka inzalo esitshalweni, sekuyisikhathi sokuqala isiko. Lokhu kuhilela ukudlulisa impahla yesitshalo esivalwe inzalo endaweni yokukhula equkethe imisoco namahomoni adingekayo ukuze kukhuliswe ukukhula nokuthuthuka. Indawo yokukhula kufanele ingabi namagciwane futhi ingabi nakungcola okungalimaza izitshalo ezintsha. Kunezinhlobo eziningi ezahlukene zemidiya yokukhula etholakalayo yesiko lezicubu, ngakho-ke khetha eyodwa elungele ukusakazwa kwe-Alocasia.

Ukuqala isiko, dlulisela ngokucophelela isitshalo esivalwe inzalo endaweni yokukhula usebenzisa amasu angenalutho. Lokhu kungase kuhlanganise ukusika isitshalo sibe izingcezu ezincane noma izitsha ukuze ukhuthaze ukukhula. Uma isitshalo sesisendaweni yokukhula, vala isitsha ngesivalo noma ifilimu ukuze udale indawo enomswakama ezokhuthaza ukukhula. Beka isitsha endaweni efudumele, ekhanyayo, kodwa ngaphandle kwelanga eliqondile, ukukhuthaza ukukhula nokuthuthuka.

I-Subculturing kanye Nokuphindaphindeka

Njengoba isitshalo sikhula endaweni yokukhula, uzodinga ukutshala kancane futhi wande izitshalo ukuze udale inzalo eyengeziwe. Lokhu kuhlanganisa ukudlulisa isitshalo esikhulayo endaweni entsha yokukhula ukuze kukhuthaze ukukhula nokuthuthuka okwengeziwe. I-Subculturing ibalulekile ukuze kugcinwe impilo namandla ezitshalo futhi kuvinjwe ukuthi zingaminyana noma zingcoliswe.

Ukuze wenze izitshalo zikhule kancane, zisuse ngokucophelela endaweni yokuqala yokukhula bese uzidlulisela endaweni entsha usebenzisa amasu oyinyumba. Qinisekisa ukuthi uhlukanisa izitshalo ngendlela efanele ukuze uvimbele ukuminyana nokungcoliswa. Njengoba izitshalo ziqhubeka zikhula futhi zikhula, ungaqhubeka nokuzithuthukisa ukuze udale inzalo eyengeziwe. Le nqubo ye-subculturing kanye nokuphindaphinda ingaphinda izikhathi eziningana ukuze kukhiqizwe inani elikhulu lezitshalo ezifanayo zofuzo ukuze zisakaze.

Ukujwayela kanye Nokudluliselwa Emhlabathini

Uma usuziphindaphinde izitshalo ngokusebenzisa isiko lamathishu, sekuyisikhathi sokuzijwayeza endaweni yazo entsha futhi uzidlulisele emhlabathini ukuze zikhule futhi zithuthuke. I-Acclimatization yisinyathelo esibalulekile enqubweni yesiko lezicubu, njengoba kusiza izitshalo ukuthi zijwayele indawo ezizungezile futhi kuvimbele ukushaqeka noma ingcindezi. Ukuze zijwayeze izitshalo, zibeke kancane kancane izinga lokuswakama eliphansi kanye nokwanda kokukhanya ukuze zizisize zishintshele endaweni yazo entsha.

Ngemuva kokujwayela izitshalo, ungazidlulisela emhlabathini ukuze zikhule futhi zithuthuke. Khetha ingxube yebhodwe egeleza kahle efanele izitshalo ze-Alocasia bese utshala ngokucophelela izitshalo ezikhuliswe izicubu. Beka izitshalo endaweni efudumele, ekhanyayo, kodwa ngaphandle kwelanga eliqondile, ukukhuthaza ukukhula nokuthuthuka. Nisela izitshalo njalo futhi uqaphe inqubekelaphambili yazo njengoba zizinza endaweni yazo entsha.

Sengiphetha, ukusakazwa kwesiko lezicubu kuyindlela ebalulekile yokwandisa izitshalo ezifana ne-Alocasia nokudala inzalo efana nofuzo. Ngokulandela lo mhlahlandlela wesinyathelo ngesinyathelo, ungakwazi ukusabalalisa ngempumelelo izitshalo ze-Alocasia ngokusebenzisa isiko lezicubu futhi ukhiqize izitshalo eziningi ezinempilo, ezingenazifo. Ukusuka ekukhetheni isitshalo esifanele ukuya ekujwayelaneni nezitshalo endaweni yazo entsha, isinyathelo ngasinye enqubweni yesiko lezicubu sibalulekile empumelelweni. Ngokuzijwayeza nangesineke, ungaba uchwepheshe ekusakazweni kwezicubu ze-Alocasia futhi ujabulele imivuzo yokukhula kwezitshalo ezinhle, ezihlukile.