Complete Guide To Sansevieria Tissue Culture Propagation

Isingeniso:

I-Sansevieria, eyaziwa nangokuthi isitshalo senyoka noma ulimi lukamamazala, isitshalo sasendlini esidumile esaziwa ngokubukeka kwaso okumangalisayo kanye nezidingo zokunakekelwa okuphansi. Ukusabalalisa isiko lezicubu kuyindlela yesimanje futhi ephumelelayo yokusabalalisa izitshalo ze-Sansevieria, okuvumela ukukhiqizwa okukhulu kwezitshalo ezifanayo nezingenazifo. Kulo mhlahlandlela ophelele, sizohlola inqubo yokusatshalaliswa kwesiko lezicubu ze-Sansevieria ngokuningiliziwe, kusukela ekukhethweni kwezitshalo kuya ekufinyeleleni kwezithombo zezitshalo ezifuywe ngezicubu.

Ukukhetha Isitshalo Somzali

Lapho uqala ukusabalalisa isiko lezicubu ze-Sansevieria, ukukhetha isitshalo esingumzali esifanele kubalulekile ukuze kube nemiphumela eyimpumelelo. Khetha isitshalo se-Sansevieria esinempilo nesingenazifo esinezici ezifiselekayo njengombala ogqamile, amahlamvu aqinile, nomkhuba wokukhula ohlangene. Isitshalo esikhethiwe sizosebenza njengomthombo wezitshalo zokuqalisa isiko lezicubu. Qinisekisa ukuthi isitshalo esingumzali asinazo izinambuzane nezifo ukuvimbela ukungcola ngesikhathi senqubo yesiko lezicubu.

Izinhlobo ze-Sansevieria ziyahlukahluka ngamaphethini azo okukhula kanye nezici zamahlamvu, ngakho-ke khetha isitshalo esiqondana nemigomo yakho yokusabalalisa. Ezinye izinhlobo ezidumile ze-Sansevieria zokusabalalisa isiko lezicubu zihlanganisa i-Sansevieria trifasciata, i-Sansevieria cylindrica, ne-Sansevieria masoniana. Uma usukhethile isitshalo somzali esifanele, sekuyisikhathi sokudlulela esinyathelweni esilandelayo senqubo yokusabalalisa isiko lezicubu.

Ukuqala I-Tissue Culture

Isigaba sokuqala sokusatshalaliswa kwesiko lezicubu ze-Sansevieria sifaka ukuqalisa isiko kusuka ezitshalweni zezitshalo. Ama-explants amasampula amathishu amancane athathwe esitshalweni esingumzali, ngokuvamile amathiphu amahlumela noma amaqabunga amancane. Lawa ma-explant afakwa inzalo ngaphezulu ukuze kuqedwe noma yimaphi ama-microorganisms angangcolisa inqubo yesiko lezicubu. Ukuvala inzalo kuyisinyathelo esibalulekile esidinga ukunemba nokunakwa kwemininingwane ukuze kuvinjelwe ukungcoliswa kanye nokuqinisekisa ukuphumelela kokuqalwa kwesiko lezicubu.

Ngemva kokuvala inzalo, izithasiselo zifakwa endaweni eyinzalo yezakhamzimba equkethe inhlanganisela yezilawuli zokukhula kwezitshalo, amavithamini, ushukela, nezinye imisoco ebalulekile. Lesi sikhungo sihlinzeka ngemisoco edingekayo ukuze izitshalo ezitshalwayo zikhule futhi zikhule zibe izimila endaweni elawulwayo. I-initiation medium ingase futhi ibe nama-gelling agents afana ne-agar ukusekela ukukhula kwezitshalo.

Shoot Ukwanda nokuphindaphinda

Uma ama-explant eseqale usiko lwezicubu futhi enze amahlumela, isigaba esilandelayo sibandakanya ukwanda kwehlumela kanye nokuphindaphindeka ukuze kwandiswe inani lezitshalo. Ukwanda kwe-Shoot kufinyelelwa ngokusebenzisa i-subculture yezitshalo zokuqala emithonjeni yezondlo ezintsha ukuze kuthuthukiswe ukukhula kwamahlumela amasha. Le nqubo ivumela ukukhiqizwa kwezitshalo eziningi ezivela endaweni eyodwa, okwandisa ukusebenza kahle kokusakazwa kwesiko lezicubu.

Ngesikhathi sokukhula kwamahlumela, kubalulekile ukuqapha ukukhula nokukhula kwezitshalo njalo. Lungisa izakhi njengoba kudingeka ukuze usekele ukukhula okuhle futhi uvimbele ukuntula izakhi noma ukungalingani. Ukuphindaphindeka kwehlumela kungathuthukiswa ngokuqhubekayo ngokulawula ukugxila kwezilawuli zokukhula kwezitshalo emithonjeni yezokudla ukuze kuthuthukiswe ukwakheka kwehlumela kanye namagatsha.

I-Rooting kanye ne-Acclimatization

Lapho ama-plantlets esedlule ukwanda nokuphindaphindeka kwehlumela, isigaba sokugcina sokusakazwa kwesiko lezicubu ze-Sansevieria sifaka ukumila kwezimpande kanye nokujwayelana. Izimpande ziwuhlelo lokunxenxa ama-plantlets ukuthi akhule izimpande, okubalulekile ukuze ziphile futhi zikhule njengezitshalo ezizimele. Ama-plantlets adluliselwa endaweni yokufaka izimpande elungiselelwe ukuthuthukiswa kwezimpande, ngokuvamile equkethe ukugxila okuphansi kwezilawuli zokukhula kwezitshalo ukukhuthaza ukukhula kwezimpande.

Ngemva kokukhula kwezimpande, ama-plantlets alungele ukujwayelana nemvelo yangaphandle. I-Acclimatization inqubo ehamba kancane ehlanganisa ukuguqula izitshalo ezitshalwe ngezicubu zisuka ezimeni ezilawulwayo zelabhu yesiko lezicubu ziye ezimweni ezizungezile zendawo yazo entsha ekhulayo. Kancane kancane veza ama-plantlets emazingeni okukhanya akhulayo, umswakama, nokugeleza komoya ukuze uzisize zijwayelane nendawo yazo entsha futhi zivikele ukushaqeka kokufakelwa.

Isifinyezo:

Ukusakazwa kwesiko lezicubu ze-Sansevieria kuyindlela ephumelelayo nethembekile yokusabalalisa izinombolo eziningi zezitshalo ezifanayo nezingenazifo. Ngokulandela izinyathelo ezivezwe kulo mhlahlandlela ophelele, ungakwazi ukusabalalisa ngempumelelo izitshalo ze-Sansevieria ngokusebenzisa isiko lezicubu, kusukela ekukhetheni isitshalo esingumzali kuye ekujwayelaneni nezimila ezikhuliswe ngezicubu. Ukusakazwa kwesiko lezicubu kunikeza izinzuzo eziningi, okuhlanganisa ukuphindaphindeka ngokushesha kwezitshalo, ukusakazeka okungenazifo, nokukhiqizwa kwezitshalo ezifanayo ngofuzo. Linga ngezinhlobonhlobo ze-Sansevieria nezimo zesiko lezicubu ukuze uthuthukise inqubo yakho yokusabalalisa futhi wandise iqoqo lakho lalezi zitshalo zasendlini ezimangalisayo. Ukuhlakulela i-Sansevieria ngokusabalalisa isiko lezicubu kuvumela ukukhiqizwa ngobuningi kwalezi zitshalo ezithandwayo, ukuqinisekisa ukutholakala okuqinile kwezibonelo ezinempilo nezinempilo zekhaya lakho noma engadini.