Intuthuko Ocwaningweni Nokuthuthukiswa Kwezicubu ze-Aglaonema

Isingeniso

I-Aglaonema, eyaziwa ngokuthi izihlahla ezihlala ziluhlaza zaseShayina, ziyizitshalo zasendlini ezidumile futhi eziguquguqukayo ezaziwa ngamahlamvu azo akhangayo. Ngezinhlobonhlobo zamaphethini nemibala yamaqabunga, izinhlobo ze-Aglaonema seziyintandokazi phakathi kwabathandi bezitshalo nabaklami bangaphakathi ngokufanayo. Kodwa-ke, ukukhiqiza kabusha lezi zitshalo ngezinga elikhulu kube yinselele ngenxa yezinga lazo lokukhula kancane kanye nokutholakala okulinganiselwe kwezitshalo ezingabazali. Ukuze banqobe le mikhawulo, abacwaningi babambe iqhaza elikhulu ocwaningweni nasekuthuthukisweni kwezicubu ze-Aglaonema, okuholele entuthukweni ebalulekile eminyakeni yamuva nje. Kulesi sihloko, sizohlola impumelelo yakamuva kusiko lwezicubu ze-Aglaonema kanye nomthelela wawo ongaba khona embonini ye-horticulture.

Ukubaluleka Kwesiko Lezicubu Ocwaningweni Lwe-Aglaonema

I-tissue culture, eyaziwa nangokuthi i-micropropagation, iyindlela ehlanganisa ukukhula kwamaseli ezitshalo, izicubu, noma izitho endaweni yaselabhorethri. Inikeza izinzuzo eziningi kunezindlela ezivamile zokusakaza, njengokuphindaphinda ngokushesha, ukutholakala unyaka wonke, izitshalo ezingenazo izifo, nokufana ekukhuleni nakwikhwalithi. Endabeni ye-Aglaonema, isiko lezicubu libonakale libalulekile ekuhlangabezaneni nesidingo esikhulayo salezi zitshalo ezithandwayo.

Ngokusebenzisa isiko lezicubu, abacwaningi bangaqala ngesiqephu esincane sezicubu zesitshalo, njengeqabunga noma i-node, bese benza amangqamuzana awo aphindaphindeka futhi ahlukanise abe izitshalo eziphelele. Le nqubo ivumela ukukhiqizwa kwezitshalo eziningi ezifanayo phakathi nesikhathi esifushane. Ukwengeza, isiko lezicubu lenza ukuba kukhethwe futhi kulondolozwe izici ezifiselekayo, njengamaphethini amaqabunga ahlukile noma ukumelana nezinambuzane nezifo, okuqinisekisa ukukhiqizwa kwezinhlobo eziphakeme ze-Aglaonema.

Ukuthuthuka Kokwakhiwa Okumaphakathi Kwesiko Lezicubu Ze-Aglaonema

Ukuphumelela kwesiko lezicubu kuncike kakhulu ekwakhiweni kwemidiya yokukhula, ehlinzeka ngemisoco edingekayo kanye nezithako zamahomoni zokukhula kwezitshalo. Ngokuhamba kweminyaka, abacwaningi baye bathuthukisa futhi bacwenga ukwakheka okuphakathi nendawo ukuze bathuthukise izinqubo zesiko lezicubu ze-Aglaonema.

Ngokwesiko, i-Murashige ne-Skoog (MS) medium, ingxube yezakhi ezisetshenziswa kakhulu, isetshenziswe ezifundweni zesiko lezicubu ze-Aglaonema. Kodwa-ke, intuthuko yakamuva igxile ekuthuthukiseni i-MS medium ngokulungisa ukwakheka kwayo nokuhlanganisa izithasiselo ukuze kuthuthukiswe ukukhula nokuthuthuka kwezitshalo.

Isibonelo, isifundo sika-Li et al. (2019) ibonise umphumela omuhle wokwengeza amalahle acushiwe ku-MS medium ukuze kuthuthukiswe ukwanda kwehlumela nokwelula e-Aglaonema. I-activated charcoal, i-porous carbon material, ithuthukisa ukumuncwa kwezakhi futhi ikhuthaze ukukhiqizwa kwama-hormone ezicutshini zezitshalo, okuholela ekukhuleni okusheshayo nokunempilo. Ukuguqulwa okunjalo kwemidiya yokukhula kunikeza amathemba ajabulisayo okwandisa ukusebenza kahle kokukhiqizwa kwesiko lezicubu ze-Aglaonema.

Iqhaza Lezilawuli Zokukhula ku-Aglaonema Tissue Culture

Izilawuli zokukhula zidlala indima ebalulekile ekulawuleni ukukhula nokuthuthuka kwezitshalo kusiko lwezicubu. Angakwazi ukugqugquzela ukuhlukana kwamangqamuzana, abangele ukwakheka kwezimpande noma ukudubula, futhi alawule ukukhiqizwa kwamahomoni ezitshalo. Kusiko lwezicubu ze-Aglaonema, ukusetshenziswa okunembile kwezilawuli zokukhula kubalulekile ukuze kuzuzwe imiphumela emihle.

Isilawuli sokukhula esisetshenziswa kakhulu kusiko lwezicubu ze-Aglaonema i-benzylaminopurine (BAP), ihomoni yokwenziwa ye-cytokinin. I-BAP ikhuthaza ukwanda kwamahlumela futhi idlala indima ebalulekile ekuqalisweni kwamahlumela amaningi avela esitshalweni esisodwa. Ocwaningweni olwenziwa nguNguyen et al. (2020), abacwaningi bathuthukise ukugxila kwe-BAP endaweni yokukhula futhi bathola izinga eliphezulu lokuphindaphinda lamahlumela e-Aglaonema.

Ngaphezu kwama-cytokinins, ama-auxins afana ne-indole-3-butyric acid (IBA) kanye ne-naphthaleneacetic acid (NAA) avame ukusetshenziswa ukuze afake izimpande emahlumela e-Aglaonema. Lawa ma-auxins akhuthaza ukwakheka kwezimpande ezifikayo, okuvumela ukuthuthukiswa kwezitshalo ezizimele ezingajwayelaniswa ngempumelelo futhi zidluliselwe emhlabathini.

Ukusetshenziswa kwe-Biotechnology Techniques ku-Aglaonema Tissue Culture