I-Aglaonema Tissue Culture Imikhuba Engcono Kakhulu: Imihlahlandlela Yokulawula Ikhwalithi Nokusabalalisa

Isingeniso:

I-Aglaonema, evame ukubizwa ngokuthi i-Chinese Evergreen, isitshalo sokuhlobisa esidumile esidume ngamahlamvu aso akhangayo kanye nokunakekelwa kalula. Njengoba izindlela zokutshala ziqhubeka nokuvela, isiko lezicubu liye lavela njengendlela ephumelela kakhulu yokusabalalisa lezi zitshalo. Lesi sihloko sihlose ukuhlinzeka ngemininingwane ebalulekile emikhubeni engcono kakhulu ehilelekile kusiko lwezicubu ze-Aglaonema, egxile ezinyathelweni zokulawula ikhwalithi nemihlahlandlela yokusabalalisa. Ngokuqonda okuphelele kwalezi zinqubo, izazi zezitshalo kanye nabathandi bezitshalo bangaqinisekisa ukusakazeka okuphumelelayo nokugcina ikhwalithi yezitshalo ze-Aglaonema.

Ukulawulwa Kwekhwalithi ku-Aglaonema Tissue Culture

Ukulawulwa kwekhwalithi kuyisici esibalulekile se-Aglaonema tissue culture, eqinisekisa ukukhiqizwa kwezitshalo ezinempilo nezinamandla. Kubandakanya uchungechunge lwezinyathelo nokuhlola ukuqapha ikhwalithi iyonke yezitshalo ezisatshalaliswa. Ake sihlole ezinye zezinqubo ezibalulekile zokulawula ikhwalithi ezisetshenziswa kusiko lwezicubu ze-Aglaonema.

Ukukhethwa Kwempahla Yokutshala Okunempilo

Isisekelo sesiko lezicubu eziphumelelayo sisekukhethweni kwezinto ezitshalwayo ezinempilo nezingenazifo. Izitshalo ezingase zihlanganise amathiphu okuhluma, ama-axillary buds, noma ama-nodal segment, aqoqwa ezitshalweni eziqinile nezingenazinambuzane. Kubalulekile ukubheka izinto ezitshalwayo ukuze uthole noma yiziphi izimpawu zezifo, njengokuguquguquka kombala, i-necrosis, noma ukukhubazeka. Ngokuqinisekisa ukusetshenziswa kwezitshalo ezingenazifo, ingozi yokudlulisela amagciwane endaweni yesiko iyancishiswa.

Izindlela Zokuhlanzeka

Ukugcina indawo eyinyumba kuyo yonke inqubo yesiko lezicubu kubalulekile ukuvimbela ukungcoliswa nokuqinisekisa ukusakazeka ngempumelelo. Amasu okuhlanza, okuhlanganisa ukusetshenziswa kwezibulala-magciwane, amathuluzi angenalutho, nezivalo zokugeleza kwe-laminar, adlala indima ebalulekile ekuqedeni ukungcola kwamagciwane. Ngaphambi kokuqala inqubo yosiko lwezicubu, wonke amathuluzi nemishini kufanele kubulawe amagciwane ngokuphelele, futhi indawo yokusebenza kufanele ibulawe amagciwane ukuze kuncishiswe ubungozi bokutheleleka.

Culture Medium Ukulungiselela

I-culture medium elungiselelwe kahle ibalulekile ekukhuleni nasekuthuthukisweni kwamasiko ezicubu ze-Aglaonema. I-medium ihlanganisa inhlanganisela yama-macronutrients, ama-micronutrients, amavithamini, izilawuli zokukhula, nama-ejenti aqinisayo. Izilinganiso ezinembile kanye namasu okulungiselela anembile ayadingeka ukuze kuqinisekiswe ukuvumelana nokukhiqiza kabusha. Izinyathelo zokulawula ikhwalithi zibandakanya ukuhlola i-pH yendawo, ukuqinisekisa ukuvala inzalo okufanele, nokuhlola ukusebenza kahle kwezithasiselo ezikhethiwe.

Ukuqapha Izimo Zemvelo

Izici zemvelo zidlala indima ebalulekile empumelelweni yokusabalalisa isiko lezicubu. Ukugcina izinga lokushisa elilungile, umswakama, nezimo zokukhanya kubalulekile ukuze kuqinisekiswe ukukhula okunamandla nokuthuthukiswa kwamasiko e-Aglaonema. Ukuqapha njalo kwalezi zici kuvumela ama-horticulturists ukuthi enze izinguquko ezidingekayo, ukuqinisekisa izimo ezifanele zenqubo yesiko lezicubu. Ubuchwepheshe obusezingeni eliphezulu, njengezinhlelo zokulawula imvelo, bungasetshenziswa ukuze kuqashwe kahle futhi kugcinwe izimo ezidingekayo.

Ukulawulwa Kwezifo Nokungcoliswa

Naphezu kokuthatha izinyathelo eziqinile zokuvimbela, ingozi yokungcoliswa nokuvela kwezifo kusiko lwezicubu ayikwazi ukuqedwa ngokuphelele. Ukusebenzisa isu eliqinile lesifo kanye nokulawula ukungcoliswa kubalulekile ukuvimbela ukulahlekelwa nokugcina ikhwalithi yezitshalo ze-Aglaonema. Ukuqapha njalo, ukutholakala ngesikhathi kwezifo noma ukungcola, kanye nezinyathelo ezifanele zokulungisa, njengokwelashwa kwe-sub-culturing noma ama-antibiotic, kubalulekile ukunqoba lezi zinselele ngempumelelo.

Imihlahlandlela Yokusabalalisa Ye-Aglaonema Tissue Culture

Ukusakazwa okuphumelelayo kwezicubu ze-Aglaonema kuncike ekulandeleni imihlahlandlela ethile namasu akhuthaza ukukhula nokuthuthukiswa kwezitshalo ezinempilo. Ake sihlole eminye imihlahlandlela eyisisekelo yokusabalalisa ehilelekile kusiko lwezicubu ze-Aglaonema.

Ukusungulwa Kwezitshalo Eziyinyumba

Isinyathelo sokuqala ekusakazeni kwesiko lezicubu ze-Aglaonema ukusungulwa kwezitshalo eziyinyumba. Izinto zokufakelwa, ezikhethwe ngokusekelwe ezilinganisweni zokulawula ikhwalithi, zibulawa amagciwane kusetshenziswa izibulala-magciwane ezifanele. Lapho isivalwe inzalo, izithombo zifakwa endaweni yesiko ngaphansi kwezimo ze-aseptic. Ukuphathwa kahle kwezichazili kuqinisekisa ukungcoliswa okuncane kanye nempumelelo enkulu esigabeni sokusungulwa.

Ukwethulwa kwama-Multiple Shoots

Ukuphindaphindeka kwamahlumela kuyisigaba esibalulekile kusiko lwezicubu ze-Aglaonema, okuvumela ukukhula ngokushesha kwezitshalo. Ngokungezwa kwezilawuli zokukhula ezishiwo, ukuthuthukiswa kwamahlumela amaningi avela ku-explants yokuqala kungenziwa. Ama-Cytokinins, ama-hormone okukhula kwezitshalo asetshenziswa kakhulu, adlala indima ebalulekile ekulawuleni ukwakheka kwamahlumela. Ngokuthuthukisa ukugcwala kwama-cytokinins endaweni yamasiko, ochwepheshe bezimbali bangakhuthaza ngempumelelo ukuphindaphindeka kwamahlumela.

I-Rooting kanye ne-Acclimatization

Uma inani elanele lamahlumela selitholiwe, isinyathelo esilandelayo ukufaka izimpande. Ukukhula kwezimpande kungafezwa ngokushintsha indlela yesiko ukuze kuqukethe ama-auxins, okugqugquzela ukukhula kwezimpande. Isigaba sokuhluma sibe sesilandelwa wukujwayela, lapho izitshalo ezinezimpande kancane kancane zichayeka ezimeni zemvelo zangaphandle. Lolu shintsho luvumela izitshalo ukuthi zijwayelane futhi zithuthukise ukumelana ngaphambi kokuba zidluliselwe endaweni yazo yokugcina yokukhula.

Ukuqina kanye Nokutshala

Ukuqina nokufakelwa kabusha kuyizigaba ezibalulekile zokulungiselela izitshalo ze-Aglaonema ezikhuliswe ngezicubu ukuze zikhule ngempumelelo futhi zithuthuke endaweni yazo yokugcina. Ngesikhathi sokuqina, izitshalo zivezwa kancane kancane ekukhanyeni okuthuthukisiwe, ukuncishiswa kwamazinga omswakama, nokwanda kokujikeleza komoya. Le nqubo iqinisa izitshalo futhi izilungiselela ukuphila ngaphandle kwelabhorethri yesiko lezicubu ezilawulwayo. Ekugcineni, ama-plantlets aqinile atshalwa emabhodweni noma emibhedeni, lapho engakwazi ukumila futhi akhule abe izitshalo ezinempilo, ezivuthiwe.

Isiphetho:

Sengiphetha, ukwazi izinqubo ezingcono kakhulu zesiko lezicubu ze-Aglaonema, ngokugxila kakhulu ekulawuleni izinga neziqondiso zokusabalalisa, kubalulekile ekusakazeni ngempumelelo nokugcinwa kwekhwalithi yezitshalo. Ukufaka izinqubo ezinjengokukhetha izinto ezisetshenziswayo ezinempilo, ukuqinisekisa ukukhucululwa kwendle okufanele, ukuqapha izimo zemvelo, ukulawula izifo nokungcola, kanye nokulandela imihlahlandlela ethile yokusabalalisa kuvumela ama-horticulturists ukuthi akhiqize izitshalo eziphakeme ze-Aglaonema. Ngokusebenzisa lezi zinqubo, imboni yesiko lezicubu ingathuthukisa ukukhiqizwa kwe-Aglaonema futhi ibe negalelo ekutholakaleni kwezitshalo ezinempilo nezinempilo kubathandi bezitshalo nabahlobisi bangaphakathi ngokufanayo.