I-Aglaonema Tissue Culture Techniques: Ukushayela Okusha Ekusakazeni Kwezitshalo

Isingeniso:

Izitshalo zidlala indima ebalulekile ekuphileni kwethu kwansuku zonke, zisinikeza umoya-mpilo, ukudla, nobuhle bobuhle. Ubuciko bokuzalanisa, noma ukuzala kabusha kwezitshalo, sebulokhu lwenziwa amakhulu eminyaka. Ngokwesiko, lokhu kwakuhilela izindlela ezinjengokuhluma kwembewu nokusikwa. Nokho, ngokuthuthuka kwezobuchwepheshe, kuye kwavela izindlela ezintsha, ezishintsha umkhakha wokusabalalisa izitshalo. Enye yalezi zindlela isiko lezicubu, eliye lazuza ukuthandwa okukhulu ngenxa yokusebenza kahle nokusebenza kahle kwalo. Kulesi sihloko, sizongena emhlabeni othakazelisayo wamasu e-Aglaonema tissue culture futhi sihlole ukuthi aqhuba kanjani ubuciko ekusakazweni kwezitshalo.

Izisekelo ZeSiko Lwezicubu

Isiko lezicubu kuyindlela ebandakanya ukukhula nokugcinwa kwamangqamuzana ezitshalo noma izicubu endaweni elawulwayo. Ivumela ukukhiqizwa kwezitshalo eziningi ezingenazo izifo kanye nezakhi zofuzo ezifanayo. I-Aglaonema, evame ukubizwa ngokuthi i-Chinese evergreen, isitshalo sokuhlobisa esidumile. Isiko lezicubu seziphenduke ithuluzi elibalulekile ekusakazeni i-Aglaonema ngenxa yezinzuzo zayo eziningi.

Enye yezinzuzo eziyinhloko zesiko lezicubu yikhono lokukhiqiza izitshalo eziningi ezivela esitshalweni esincane sesitshalo. Lokhu kwaziwa ngokuthi yi-micropropagation futhi kubandakanya ukuphindaphindeka kwamahlumela, izimpande, noma imibungu endaweni yaselabhorethri. Inqubo iqala ngokukhethwa kwesitshalo esinempilo nesingenazifo, lapho kukhishwa khona ucezu oluncane lwethishu noma isitshalo. Lesi sitshalo sibe sesidluliselwa endaweni yokukhula equkethe izakhamzimba ezibalulekile, amavithamini, namahomoni ezitshalo. Ngaphansi kwezimo ezilawulwayo, i-explant idlula ukuhlukaniswa kwamaseli futhi yakha i-callus, okuyinqwaba yamaseli angahlukanisiwe. Kusukela kule callus, kwakheka amahlumela amasha noma izithombo, ezingahle zijwayeleke futhi ekugcineni zidluliselwe ku-greenhouse noma insimu ukuze zikhule.

Intuthuko ku-Aglaonema Tissue Culture Techniques

Eminyakeni yamuva nje, kuye kwenziwa intuthuko enkulu kumasu e-Aglaonema tissue culture, okuthuthukisa ukusebenza kahle nempumelelo kokusakazwa kwezitshalo. Le ntuthuko ibandakanya ukwethulwa kwemithombo yezindaba yokukhula inoveli, amasu okuguqula izakhi zofuzo, kanye nokusetshenziswa kwama-bioreactors.

Iqhaza Lezindaba Zokukhula Inoveli

Ukukhethwa kwemidiya yokukhula kuthonya kakhulu impumelelo yesiko lezicubu ze-Aglaonema. Ngokwesiko, i-Murashige ne-Skoog (MS) medium isetshenziswa kakhulu. Kodwa-ke, abacwaningi bebehlola enye imidiya yokukhula ukuze bathuthukise inqubo yokusabalalisa. Esinye salezo zibonelo i-Gamborg's B5 medium, etholwe ikhuthaza ukuphindaphindeka kwehlumela okuphakeme kanye nokwelula kusiko lwezicubu ze-Aglaonema. I-B5 medium iqukethe ukuhlangana okulinganiselwe kwezakhi zezitshalo ezibalulekile, amavithamini, nezinhlanganisela eziphilayo ezisekela ukukhula nokuthuthuka okuphelele. Ukwengeza, ukufakwa kwamalahle acushiwe phakathi nendawo kubonise ukuthuthukisa ukuvuselelwa kwehlumela kanye nokuhluma kwezimpande okulandelayo.

Izinhlobonhlobo eziningi zemidiya yokukhula, njengengxenye yamandla e-MS medium and modified B5 medium, iye yathuthukiswa futhi yahlolelwa isiko lezicubu ze-Aglaonema. Le ntuthuko emithonjeni yezindaba yokukhula ayithuthukisanga izinga lokuphindaphinda kuphela kodwa futhi idlale indima enkulu ekwehliseni izindleko zokukhiqiza.

Ukuguqulwa Kwezofuzo ku-Aglaonema

Ukuguqulwa kwezakhi zofuzo kuhilela ukwethula izakhi zofuzo zangaphandle ku-genome yesitshalo, okuholela ekuvezeni izici ezifiselekayo. Le nqubo inamandla amakhulu emkhakheni wezolimo, okwenza ukuba izitshalo zikwazi ukumelana nezinambuzane, izifo kanye nezinto ezicindezela imvelo. Kusiko lwezicubu ze-Aglaonema, izindlela zokuguqula izakhi zofuzo zisetshenziswe ngempumelelo ukwethula izakhi zofuzo zokumelana nokhula, ukuguqulwa kombala wezimbali, kanye nezici ezithuthukisiwe zamahlamvu.

Inqubo yokuguqulwa kwezakhi zofuzo kusiko lezicubu ze-Aglaonema ngokuvamile iqala ngokwakhiwa kofuzo oluqukethe isakhi sofuzo esithandwayo. Lesi sakhiwo sibe sesingeniswa ku-Agrobacterium tumefaciens, igciwane elivame ukusetshenziswa njenge-vector. Ukuguqulwa kwe-Agrobacterium-mediated kubandakanya ukutshalwa ngokubambisana kwe-Aglaonema explant ne-Agrobacterium eguquliwe, okuvumela ukudluliswa kwesakhi sofuzo esifiselekayo kumaseli esitshalo. Amaseli aguquliwe abe esekhethwa futhi aphindaphindwe kusetshenziswa imidiya ekhethiwe equkethe ama-antibiotics noma imithi yokubulala ukhula. Ukuvuselelwa okulandelayo kwezitshalo kusuka kulawa maseli ashintshiwe kukhiqiza izitshalo ze-Aglaonema ezinezici ezifiselekayo.

Ukuguqulwa kofuzo kunamandla amakhulu okuthuthukiswa kwezinhlobo ze-Aglaonema ezinoveli ezinezici ezithuthukisiwe. Ivumela abalimi bezitshalo ukuba bethule izici ezifiselekayo ngokushesha nangempumelelo, baguqule inqubo yokusabalalisa izitshalo.

Ukusebenzisa ama-Bioreactors

Ama-Bioreactors ayizindawo ezilawulwayo ezihlinzeka ngezimo zokukhula ezifanele zokusabalalisa isiko lezicubu. Lezi zinhlelo zingazenzakalela kakhulu, okuvumela ukulawula okunembile kuzici zemvelo ezifana nezinga lokushisa, ukukhanya, nokutholakala kwezakhi. Ama-Bioreactors asetshenziswe ngempumelelo kusiko lwezicubu ze-Aglaonema, okuvumela ukukhiqizwa kwezitshalo ezinkulu ezinekhwalithi engaguquki nokufana.

Olunye uhlobo lwe-bioreactor oluvame ukusetshenziswa kusiko lwezicubu isistimu yokucwilisa yesikhashana (TIS). Lolu hlelo lubandakanya ukucwiliswa kwezitshalo endaweni yokukhula okuwuketshezi isikhathi esifushane, okulandelwa ukugeleza kwamanzi ukuvumela ukushintshaniswa kwegesi. Imijikelezo yokucwiliswa kanye nokukhipha amanzi ilingisa indawo yemvelo, ikhuthaza ukumuncwa okungcono kwezakhi kanye nokukhula okuthuthukisiwe. Ukusetshenziswa kwe-TIS kusiko lwezicubu ze-Aglaonema kubonise imiphumela ethembisayo, kunciphisa kakhulu isikhathi sokuphindaphinda nokwandisa izinga lokukhiqiza.

Olunye uhlobo lwe-bioreactor oluthola ukuduma kusiko lwezicubu ze-Aglaonema yi-mist bioreactor. Lolu hlelo lusebenzisa inkungu encane noma inkungu ukunikeza umswakama kanye nezakhamzimba ezitshalweni. I-Mist bioreactor idala indawo enomswakama futhi elawulwayo, ivimbele ukuphelelwa amanzi emzimbeni futhi ikhuthaze ukukhula okusheshayo. Ukusetshenziswa kwama-bioreactors enkungu kube nesandla ekukhiqizweni ngobuningi kwezitshalo ze-Aglaonema ezinekhwalithi enhle kakhulu namazinga okusinda.

Isifinyezo

Amasu e-Aglaonema tissue culture aguqule insimu yokusabalalisa izitshalo. Ngokusetshenziswa kwemithombo yezindaba yokukhula yenoveli, amasu okuguqula izakhi zofuzo, nama-bioreactors, abacwaningi nabatshali bezitshalo bakwazile ukuthuthukisa ukusebenza kahle nempumelelo kokusakazwa kwe-Aglaonema. Isiko lezicubu livumela ukukhiqizwa kwezitshalo eziningi ezingenazo izifo kanye nezakhi zofuzo, okuhlinzeka ngesixazululo esimeme sokuhlangabezana nesidingo esikhulayo sezitshalo zokuhlobisa.

Ngokuthuthuka okuqhubekayo kumasu osiko lwezicubu, singalindela ukubona ezinye izinto ezintsha ekusakazweni kwezitshalo, okuvumela ukuthuthukiswa kwezinhlobo ezintsha ze-Aglaonema ezinezici ezithuthukisiwe kanye nokwanda kokumelana nezingcindezi zemvelo. Ikusasa le-Aglaonema tissue culture linesithembiso esikhulu kubo bobabili abathanda izitshalo kanye nabatshali bezentengiselwano ngokufanayo, okugqugquzela ukuqanjwa okusha ekusakazeni lesi sitshalo esithandwayo sokuhlobisa.