Isingeniso
Isiko lama-tissue liba yindlela ethandwa kakhulu yokutshala izitshalo ngenxa yezinzuzo zayo eziningi. Le ndlela ivumela ukuzalanisa izitshalo ngaphansi kwezimo eziyinyumba, iqinisekisa izibonelo ezingenazo izifo kanye nezinga eliphezulu lokusinda. Kodwa-ke, ukunakekela izitshalo zesiko lezicubu kudinga ulwazi oluthile namasu ukuze kuqinisekiswe ukukhula nokuthuthuka kwazo okufanele. Kulesi sihloko, sizohlola izinto ezibalulekile zokunakekela izitshalo zesiko lamathishu, kusukela esigabeni sokuqala sokujwayela ukuya ekunakekeleni isikhathi eside.
Isigaba Sokujwayela
Ngesikhathi sesigaba sokujwayela, izitshalo ze-tissue culture ziyashintsha zisuka endaweni elawulwayo yelabhorethri ziye ezimweni zangaphandle ezizobe zikhula kuzo. Lesi sigaba sibalulekile ukusiza izitshalo ukuthi zizijwayeze futhi zichume endaweni yazo entsha. Izinyathelo ezilandelayo zibalulekile ukuze kufinyeleleke ngempumelelo:
Ukushintsha Kancane Kancane: Izitshalo zokukhiqiza izicubu kudingeka zivezwe kancane kancane ezimweni zangaphandle ukugwema ukushaqeka. Le nqubo, eyaziwa ngokuthi ukuqina, ihlanganisa ukunciphisa umswakama ohlobene nokwehlisa izinga lokushisa ngendlela elawulwayo. Lokhu kuguquka kancane kancane kuvumela izitshalo ukuthi zivumelane namazinga okukhanya avamile kanye nokwehluka kwezinga lokushisa.
Izinga Lokushisa Elilungile Nomswakama: Gcina izinga lokushisa elifanele kanye nezinga lokushisa ngesikhathi sokujwayela. Ezitshalweni eziningi zesiko lezicubu, izinga lokushisa elingu-20-25°C (68-77°F) kanye nomswakama ohlobene no-60-70% lilungile. Gada la mapharamitha eduze futhi wenze izinguquko njengoba kudingeka.
Ukungena komoya Okufanelekile: Ukugeleza komoya okuhle kubalulekile ukuze kuvinjwe ukunqwabelana komswakama owedlulele futhi kukhuthaze ukushintshaniswa kwamagesi. Qinisekisa ukuthi indawo ekhulayo ingenisa umoya omuhle ngokusebenzisa amafeni noma izindlela zokungenisa umoya zemvelo. Lokhu kusiza ukuvimbela ukuthuthukiswa kwezifo zokukhunta noma zebhaktheriya ezikhula kahle emoyeni omile.
Ukwehliswa Kancane Kancane Kokukhanya Okungenziwanga: Izitshalo ze-tissue culture zijwayele ukukhanya kokwenziwa okunamandla aphezulu elabhorethri. Kancane kancane nciphisa amandla okukhanya esikhathini esiyizinsuku noma amasonto ukuze usize izitshalo zijwayelane nezimo zokukhanya kwemvelo. Lokhu kuncipha kancane kancane kunciphisa ingcindezi futhi kuvumela izitshalo ukuthi zilungise umthamo wazo we-photosynthetic.
Ukuvikelwa Ezinambuzaneni Nezifo: Ngesikhathi sesigaba sokujwayela, izitshalo ze-tissue culture zisengozini enkulu yezinambuzane nezifo. Sebenzisa izindlela zokuvimbela, njengokuhlola okuvamile, izinqubo ezifanele zokukhucululwa kwendle, kanye nokusetshenziswa kwemithi yokwelapha ephilayo noma yamakhemikhali lapho kudingeka. Lokhu kuzosiza ukuvikela izitshalo zakho futhi kuvimbele ukwethulwa kwamagciwane endaweni yakho ekhulayo.
Ukugcinwa Kwesikhathi Eside
Uma izitshalo ze-tissue Culture sezijwayele ngempumelelo, zidinga ukunakekelwa okukhethekile ukuze kuqinisekiswe ukukhula kwazo kwesikhathi eside nempilo. Landela le mihlahlandlela ukuze ulondoloze ngempumelelo:
I-Substrate kanye Ne-Potting: Khetha i-substrate efanelekile noma ingxube yobumba yezitshalo zakho zesiko lamathishu. Ukukhethwa kwe-substrate kuncike ezinhlobonhlobo zezitshalo kanye nezidingo zayo ezithile. Kufanele inikeze ukugeleza okwanele, ukungena nomoya, nokugcinwa kwezakhi. Cabanga ngengxube ye-peat moss, i-perlite, i-vermiculite, ne-organic matter ukuze uthole imiphumela emihle.
Ukunisela: Ukunisela ngendlela efanele kubalulekile ukuze kugcinwe izitshalo zesiko lezicubu. Gwema ukuchelela ngokweqile, ngoba kungaholela ekulimaleni kwezimpande nezinye izindaba ezihlobene nomswakama. Qinisekisa ukuthi i-substrate inomswakama kancane kodwa ayinamanzi. Ukuvama kokunisela kuncike ezintweni ezifana nohlobo lwesitshalo, ukwakheka kwe-substrate, nezimo zemvelo. Gada izinga lomswakama njalo ukuze ugweme ukweqisa.
Ukuvundisa: Izitshalo zokukhiqiza izicubu zinezidingo eziphezulu zomsoco ngenxa yezinga lokukhula ngokushesha. Sebenzisa umanyolo olinganiselayo, okhipha kancane ukuze unikeze imisoco ebalulekile. Landela imiyalelo yomkhiqizi wohlelo olufanele kanye nomthamo. Gada njalo amahlamvu ukuze uthole izimpawu zokushoda kwezakhi noma ukweqisa futhi ulungise ukuvundisa ngendlela efanele.
Izidingo Zokukhanya: Ukuqonda izidingo zokukhanya zezitshalo zakho ezithinta izicubu kubalulekile ekukhuleni nasekuthuthukeni kwazo. Izinhlobo zezitshalo ezihlukene zinezinketho ezihlukene zokuqina kokukhanya nobude besikhathi. Beka izitshalo zakho endaweni lapho zingathola khona ukukhanya okufanele. Ukukhanyisa okungeziwe kungase kudingeke, ikakhulukazi ezimweni zokukhanya okuphansi noma phakathi nezinkathi ezithile zonyaka.
Ukuthena Nokunakekela: Ukuthena nokugcinwa njalo kubalulekile empilweni nasekubukekeni kwezitshalo ezithuthukisa izicubu. Susa noma yiziphi izitshalo ezifile, ezilimele, noma ezigulayo ukuze uvimbele ukusabalala kwezifo. Ukwengeza, ukuthena kusiza ukugcina isimo esifiselekayo nobukhulu bezitshalo. Sebenzisa amathuluzi ahlanzekile futhi acwengekile uma wenza imisebenzi yokuthena ukuze unciphise ubungozi bokungenisa amagciwane.
Isifinyezo
Ukunakekela izitshalo zesiko lezicubu kudinga ukunakwa kwemininingwane kanye nokunamathela kumasu athile. Isigaba sokujwayelana sibalulekile ekusizeni izitshalo ukuthi zivumelane nezimo zangaphandle, kuyilapho ukunakekelwa kwesikhathi eside kuqinisekisa ubungqabavu bazo obuqhubekayo. Ngokushintsha izitshalo kancane kancane, unikeze izimo zemvelo ezilungile, nokusebenzisa izindlela zokunakekela ezifanele, ungajabulela izitshalo ezinempilo nezichumayo zezicubu. Khumbula ukuhlala uqaphile ngokumelene nezinambuzane nezifo, khetha ama-substrates afanelekile, futhi uhlangabezane nezidingo zokukhanya nezomsoco zezitshalo zakho. Ngalezi zinyathelo ezikhona, uzoklonyeliswa ngezitshalo ezinhle neziqinile zezicubu ezingadini noma endaweni yasendlini.
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