Ungalikhulisa kanjani i-Tissue Culture Anthurium?

Izitshalo ze-Anthurium zaziwa ngezimbali zazo ezimangalisayo, ezimise okwenhliziyo namaqabunga acwebezelayo, aluhlaza ngokumnyama. Ukuhlakulela isiko lezicubu ze-Anthurium kuyinqubo ethakazelisayo evumela ukuthi usakaze lezi zitshalo ezinhle ngezinga elikhulu. Ngokusebenzisa amasu wosiko lwezicubu, ungakha izitshalo eziningi ze-Anthurium esitshalweni esinomzali oyedwa, uqinisekise ukufana nokuvumelana ezitshalweni zakho. Kulesi sihloko, sizocubungula imininingwane yokuthi singatshala kanjani ngempumelelo isiko lezicubu i-Anthurium.

Ukuqonda Isiko Lwezicubu

Isiko lezicubu kuyindlela esetshenziselwa ukusabalalisa izitshalo kusetshenziswa izingcezu ezincane zezicubu zezitshalo, njengamathiphu esiqu noma izingxenye zamaqabunga, endaweni eyinyumba ecebile ngezakhi. Le nqubo ivumela amaseli ezitshalo ukuba ahlukane futhi akhule abe isitshalo esiphelele ngaphansi kwezimo ezilawulwayo. Endabeni ye-Anthurium, isiko lamathishu linenzuzo ikakhulukazi njengoba lenza ukukhiqizwa kwenani elikhulu lezitshalo ezifanayo ngesikhathi esifushane. Le ndlela iwusizo ikakhulukazi kubalimi bezentengiselwano abafuna ukukhiqiza ngobuningi izitshalo ze-Anthurium ezithengiswayo.

Ukuqala inqubo yesiko lezicubu, inani elincane lezicubu zesitshalo, elibizwa ngokuthi i-explant, lithathwa esitshalweni esinempilo se-Anthurium. I-explant ibe isivalwa inzalo ukuze kuqedwe noma yikuphi ukungcola okungase kuvimbe ukukhula kwesiko lezicubu. Uma isitshalo sesifakwe inzalo, sifakwa endaweni enezakhi eziqukethe imisoco ebalulekile yezitshalo, amavithamini kanye nezilawuli zokukhula. Lezi zingxenye zikhuthaza ukukhula nokuthuthukiswa kwamangqamuzana ezitshalo, ziwavumela ukuba enze amahlumela, izimpande, futhi ekugcineni, isitshalo esiphelele.

Isiko lezicubu linikeza izinzuzo ezimbalwa kunezindlela zendabuko zokusabalalisa izitshalo. Okokuqala, ivumela ukukhiqizwa okusheshayo kwezitshalo eziningi ezivela esitshalweni esinomzali oyedwa, ukuqinisekisa ukufana kwezakhi zofuzo kanye nokuvumelana phakathi kwenzalo. Ukwengeza, isiko lezicubu lingasiza ekuqedeni izifo nezinambuzane ezingase zibe khona esitshalweni esingumzali, njengoba le nqubo ihilela ukuvala inzalo okuphelele kwesitshalo. Sekukonke, isiko lezicubu kuyindlela ebalulekile yokusabalalisa izitshalo ze-Anthurium ngokuphumelelayo nangempumelelo.

Ukusetha ilebhu yesiko lezicubu

Ngaphambi kokuthi uqale ukutshala isiko lezicubu ze-Anthurium, uzodinga ukumisa ilebhu yesiko lezicubu ngemishini edingekayo kanye nempahla. Ilebhu ye-tissue Culture kufanele ibe indawo ehlanzekile, eyinyumba engenazo izinto ezingcolile ezingase zibe sengozini ukukhula kwezicubu zesitshalo. Ezinye izinto ezibalulekile nezinto ezisetshenziswa zelabhu yesiko lezicubu zihlanganisa ama-laminar flow hoods omsebenzi oyinyumba, imikhumbi yamasiko efana nezitsha zePetri namashubhu okuhlola, imidiya yezakhi, izilawuli zokukhula kwezitshalo, kanye nemishini yokubulala inzalo.

Lapho usetha ilebhu yakho yesiko lezicubu, kubalulekile ukulandela imithetho efanele yokuvala inzalo ukuze uvimbele ukungcoliswa kwezicubu zesitshalo. Geza amagciwane kuwo wonke amathuluzi nezindawo usebenzisa isibulala-magciwane esifana ne-bleach noma i-ethanol ngaphambi kokuqala umsebenzi wokwenza izicubu. Ukwengeza, gcina amasu e-aseptic kuyo yonke inqubo yesiko lezicubu ukuqinisekisa ukuthi akukho ukungcola okungena emithanjeni yesiko futhi kuthinte ukukhula kwezicubu zesitshalo. Ngokusungula ilebhu yesiko lezicubu ezihlonyiswe kahle futhi enakekelwa kahle, ungakha indawo ekahle yokuhlakulela ngempumelelo isiko lezicubu i-Anthurium.

Ukulungiselela I-Explant

Isinyathelo sokuqala sokuhlakulela isiko lezicubu ze-Anthurium silungiselela ukufakelwa, noma ucezu oluncane lwezicubu zezitshalo ezizosetshenziselwa ukuqalisa isiko lezicubu. Isitshalo singathathwa ezingxenyeni ezahlukahlukene zesitshalo se-Anthurium, njengethiphu lehlumela, iqabunga, noma isiqu. Lapho ukhetha isimila, khetha isifanekiso esinempilo, esingenazifo esingenawo yimuphi umonakalo obonakalayo noma amabala. Lokhu kuzoqinisekisa ukuqaliswa ngempumelelo kwesiko lezicubu kanye nokukhula kwamaseli ezitshalo ezinempilo.

Uma usukhethile isimila, kubalulekile ukusivala amagciwane ukuze uqede noma yikuphi ukungcola okungaba khona. Ukuvala inzalo kungafinyelelwa ngenhlanganisela yezindlela ezingokwenyama nezamakhemikhali, njengokugeza isitshalo ngesisombululo sokuhlanza, okulandelwa ukucwiliswa ku-bleach noma isixazululo se-ethanol. Ngemva kokuvala inzalo, isitshalo siyasikwa sibe usayizi kanye nesimo esifunekayo, silungele ukufakwa endaweni yezakhi ukuze kuqaliswe isiko lezicubu.

Ukulungiselela ukufakelwa kuyisinyathelo esibalulekile enqubweni yesiko lezicubu, njengoba kunquma impumelelo yokusabalalisa isiko lezicubu. Ngokukhetha isimila esinempilo, esingangcolisiwe futhi ulandele izindlela ezifanele zokuvala inzalo, ungaqinisekisa ukuqaliswa ngempumelelo nokukhula kwesiko lezicubu i-Anthurium.

Ukuqala I-Tissue Culture

Uma ukufakelwa sekulungisiwe futhi kwenziwa inzalo, sekumi ngomumo ukuthi kuqaliswe isiko lezicubu. I-explant ibekwe endaweni yezakhi eziqukethe imisoco ebalulekile yezitshalo, amavithamini, nezilawuli zokukhula ezikhuthaza ukukhula nokuthuthukiswa kwamangqamuzana esitshalo. Umkhumbi we-tissue culture, ongaba isitsha sePetri noma ishubhu lokuhlola, ube usuvalwa ukuze ulondoloze indawo eyinyumba futhi ubekwe egumbini lokukhula elilawulwayo elinezinga lokushisa elilungile, umswakama, nezimo zokukhanya.

Phakathi nesigaba sokuqala sosiko lwezicubu, isimila sizoqala ukukhula futhi sikhule sibe izimila ezincane. Ukukhula kwamaseli ezitshalo kugadwa njalo ukuze kuqinisekiswe ukuthi anempilo futhi awangcoliswanga. Njengoba izimila zikhula, zingase zidinge ukukhuliswa kancane endaweni yezakhi ezintsha ukuze kube lula ukukhula nokuthuthuka. Le nqubo iyaphindwa kuze kube yilapho izitshalo zezitshalo zifinyelela usayizi owanele wokudluliselwa emhlabathini ukuze zikhule futhi zivuthwe.

Ukuqala isiko lezicubu i-Anthurium kudinga isineke nokuqapha ngokucophelela ukuze kuqinisekiswe ukukhula okuphumelelayo kwezitshalo. Ngokunikeza amangqamuzana esitshalo ngezakhi ezidingekayo kanye nabalawuli bokukhula endaweni elawulwayo, ungakhuthaza ukuthuthukiswa kwezitshalo ezinempilo, eziqinile ze-Anthurium ngokusebenzisa isiko lezicubu.

Ukudlulisa Izitshalo Emhlabathini

Uma izitshalo ezikhule ngosiko lwezicubu sezifinyelele usayizi owanele, sezilungele ukudluliselwa emhlabathini ukuze zikhule futhi zivuthwe. Ngaphambi kokudlulisela izitshalo emhlabathini, kubalulekile ukuzijwayeza endaweni yazo entsha kancane kancane. Le nqubo, eyaziwa ngokuthi ukuqina, ihilela ukubeka izitshalo emazingeni akhulayo okukhanya, izinga lokushisa, kanye nomswakama phakathi nesikhathi esithile ukuze zilungiselele ukuphila ngaphandle komkhumbi we-tissue culture.

Ukufaka ama-plantlets enhlabathini, lungiselela ingxube ye-potting efanelekile izitshalo ze-Anthurium, njengendawo yokukhipha kahle, i-peat-based medium. Yenza imbobo encane enhlabathini futhi ususe ngokucophelela isitshalo esitsheni se-tissue culture, uqaphele ukuthi ungalimazi izimpande ezithambile. Faka kahle isitshalo embobeni bese uvala izimpande nomhlabathi, uqinisekise ukuthi isitshalo sivikelekile futhi siqondile. Nisela i-plantlet kancane ukuze ukhuthaze ukukhula kwezimpande futhi uyibeke endaweni efudumele, enomswakama enokukhanya kwelanga okungaqondile.

Ukudlulisa izimila ezitshalwe ngosiko lwezicubu ziye enhlabathini kuyisinyathelo esibalulekile enqubweni yokutshala, njengoba kuphawula uguquko olusuka endaweni eyinyumba yesiko lezicubu ukuya ezimweni ezikhulayo zesitshalo. Ngokuzijwayeza izitshalo kancane kancane futhi uzinikeze ukunakekelwa okudingekayo nokunakwa, ungaqinisekisa ukukhula okuphumelelayo nokuthuthukiswa kwesiko lezicubu ze-Anthurium emhlabathini.

Ekuphetheni, ukuhlakulela isiko lezicubu ze-Anthurium kuyinqubo evuzayo nenenzuzo evumela ukuthi usakaze lezi zitshalo ezinhle ngokuphumelelayo nangempumelelo. Ngokuqonda izimiso zesiko lamathishu, ukusetha ilebhu yesiko lezicubu ezifanele, ukulungiselela ukufakelwa, ukuqalisa isiko lezicubu, nokudlulisa izitshalo emhlabathini, ungakwazi ukusabalalisa ngempumelelo izitshalo ze-Anthurium ngokusebenzisa isiko lezicubu. Ngokunakekelwa okufanele nokunaka, ungajabulela isivuno esiningi sezitshalo ezinempilo, ezifanayo ze-Anthurium ezitshalwe ngesiko lezicubu. Qala uhambo lwakho lokutshala i-Anthurium namuhla futhi ujabulele injabulo yokusabalalisa lezi zitshalo ezinhle ekhaya lakho noma engadini yakho.