I-Mastering Tissue Culture Philodendron: Amasu Okusakaza Nezinzuzo

Isingeniso

Ama-Philodendron ayizitshalo zasendlini ezidumile ezaziwa ngamahlamvu azo aluhlaza kanye nezidingo zokunakekelwa okuphansi. Izindlela zokusabalalisa isiko lezicubu ziguqule indlela esiphindaphinda ngayo futhi sikhulise lezi zitshalo ezinhle. Kulesi sihloko, sizohlola umhlaba we-tissue culture Philodendron futhi sihlole amasu athakazelisayo nezinzuzo ezinikezayo. Noma ngabe ungumthandi wezitshalo ongumakadebona noma umlimi osaqala, lesi sihloko sizokunikeza imininingwane ebalulekile ngobuciko bokufunda isiko lezicubu i-Philodendron.

Okuyisisekelo kwe-Tissue Culture Philodendron

I-tissue culture isho inqubo yokutshala izitshalo kusuka kumasampula ezicubu ezincane ngaphansi kwezimo ezingenalutho, okuvumela ukusabalala ngobuningi. I-Philodendron, isitshalo esishisayo esihlala emahlathini emvula aseCentral naseNingizimu Melika, ifakazele ukuthi iyikhandidethi elihle kakhulu lokusabalalisa isiko lezicubu. Le nqubo ihlanganisa ukuhlukanisa ucezu oluncane lwezicubu zesitshalo, ngokuvamile ihlumela le-axillary noma ithiphu lehlumela, bese ulibeka endaweni eyinyumba equkethe izakhamzimba ezibalulekile namahomoni. Ngaphansi kwezimo ezilawulwayo, amasampula ezicubu akhula abe izimila eziphelele, ezilungele ukudluliselwa enhlabathini noma ezinhlelweni ze-hydroponic.

Ama-philodendron e-tissue culture anikeza izinzuzo ezimbalwa kunezindlela zendabuko zokusakaza. Okokuqala, le nqubo ivumela ukukhiqizwa kwezitshalo eziningi ezifanayo ngesikhathi esifushane. Kuphinde kuqede ubungozi bokudluliswa kwezifo kanye nokuba khona kwezinambuzane ezivame ukuhlotshaniswa nesitokwe senkulisa yendabuko. Ukwengeza, isiko lezicubu liqinisekisa ukuthi inzalo igcina izici ezifiselekayo zesitshalo esingumzali, njengombala wamaqabunga ogqamile, amaphethini amahlamvu ahlukile, noma ukumelana nezifo.

Inqubo ye-Tissue Culture

Ukuze siqonde ngokugcwele isiko lezicubu ze-Philodendron, ake sihlole inqubo yesinyathelo nesinyathelo ehilelekile ekusakazeni lezi zitshalo ezinhle kakhulu:

Iqoqo Lesampula Lezicubu:

Isinyathelo sokuqala ekusakazweni kwesiko lezicubu ukukhetha ngokucophelela isampula yethishu. Kukhethwa isitshalo sikamama esinempilo, futhi imiqumbe ye-axillary noma amathiphu wokuhluma aqoqwa ngamathuluzi avalelwe inzalo ukuze kuncishiswe ubungozi bokungcola.

Ukuvala inzalo:

Amasampula ezicubu eziqoqiwe abe esefakwa ekubulaweni inzalo okugcwele kwendawo ukuze kuqedwe noma yimaphi ama-microorganisms angase akhinyabeze ukukhula kwawo. Lokhu ngokuvamile kufezwa ngokwelapha amasampula ngengxube yezibulala-magciwane, okulandelwa ukugeza ngamanzi angenalutho.

Ukuqaliswa:

Uma amasampula ezicubu esenziwe inzalo, adluliselwa endaweni eqinile enomsoco noma ewuketshezi, eyaziwa ngokuthi imidiya yesiko noma ijeli ye-agar. Lesi sikhungo sihlinzeka ngemisoco edingekayo, amavithamini, nezilawuli zokukhula ezibalulekile ekuhlukaniseni amaseli nokuthuthukiswa kwezitshalo.

Ukusabalala kweShoot:

Ngaphansi kwezimo ezilawulwayo - ngokuvamile egumbini lokukhula noma indawo ebamba ukushisa efakwe ukukhanya okufanele nokulawula izinga lokushisa - amasampula ezicubu aqala ukwenza imiqumbe namahlumela amancane. Lawa amahlumela aphindaphindeka ngokushesha, okuholela ekubeni iqoqo lezitshalo ezi-micropropagated.

Izimpande:

Uma isibalo esanele samahlumela sesithuthukile, ahlukaniswa ngokucophelela namaqoqo futhi adluliselwe endaweni entsha yesiko noma ingxube ye-hormone yezimpande. Lokhu kukhuthaza ukumila kwezimpande, okuholela ekwakhekeni kwesistimu yezimpande ethuthuke kahle.

Ukujwayela:

Ngemuva kokukhula kwezimpande, ama-plantlets kancane kancane ajwayelana nezimo ezingezona inyumba. Lokhu kuhlanganisa ukuwabeka emazingeni akhulayo omswakamo nokukhanya kuyilapho kunciphisa umswakama endaweni ekhulayo. Le nqubo ivumela izitshalo ukuthi zakhe ungqimba lwe-cuticle emaqabunga awo, okuwenza amelana kakhulu nokuguquguquka komswakama kanye nezinga lokushisa.

Izinzuzo ze-Tissue Culture Philodendron

Ukusakazwa kwesiko lezicubu kunikeza izinzuzo eziningi kubo bobabili abalimi bezentengiselwano nabathandi bezitshalo. Ake sihlole ezinye zezinzuzo ezizilethayo ekutshalweni kwe-Philodendron:

Ukukhiqizwa Okuningi:

Enye yezinzuzo eziyinhloko zesiko lezicubu iPhilodendron yikhono lokukhiqiza izitshalo eziningi ngesikhathi esifushane. Lokhu kusebenza kahle kusiza ukuhlangabezana nesidingo esikhula njalo salezi zitshalo ezinhle zamahlamvu emakethe.

Ukuqedwa kwegciwane kanye nezifo:

Izitshalo ze-tissue culture zitshalwa ngaphansi kwezimo ezingenalutho, ukuqinisekisa ukuthi azikho kumagciwane avamile kanye nezifo ezingaphazamisa ukukhula kwazo kanye nobuhle. Lesi sitokwe esingenazifo siqeda isidingo sezibulala-zinambuzane ezingaba yingozi noma amakhemikhali, sikhuthaze indlela yokukhula enempilo nesimeme.

Ukonga Nokulondolozwa:

Amasu okukhiqiza izicubu nawo adlala indima ebalulekile ekongiweni nasekulondolozweni kwezinhlobo ze-Philodendron ezingavamile noma ezisengozini yokushabalala. Ngokuphindaphinda okulinganiselwe kwezitshalo, isiko lezicubu liqinisekisa ukulondolozwa kwezinhlobonhlobo zofuzo futhi linomthelela emizamweni yokulondoloza lezi zitshalo ezinhle.

Ukufana Kwezitshalo:

I-tissue culture iqinisekisa ukukhiqizwa kwenzalo efana nofuzo eyaziwa ngokuthi ama-clones. Lokhu kudala iqoqo elifanayo lezitshalo, zonke zibonisa izici ezifanayo ezifiselekayo, njengombala wamaqabunga, umumo, noma amaphethini okuhlukahluka. Lokhu kuzuzisa kakhulu ezinkulisa zezentengiselwano kanye nezinkampani zemvelo zangaphakathi ezifuna izitshalo ezingaguquki nezibukeka kahle.

Ukutholakala Konyaka Wonke:

Ngokungafani nezindlela zendabuko zokusabalalisa ezingase zikhawulelwe ezinkathini ezithile zonyaka, isiko lezicubu ze-Philodendron livumela ukukhiqizwa kwezitshalo unyaka wonke. Lokhu kunikezwa kwezitshalo okuqhubekayo kuzuzisa abalimi nabathengi, kuqinisekisa ukutholakala okungaguquki emakethe.

Ukuhlola Izinhlobonhlobo Ezintsha:

Amasu osiko lwezicubu anika amandla abacwaningi nabatshali ukuthi bahlole futhi bathuthukise izinhlobo ezintsha nezithuthukisiwe ze-Philodendron. Ngokuhlukanisa izici ezifiselekayo nokusebenzisa izindlela zesiko lezicubu, abalimi bangakha izimila ezihlukile nezihehayo ezengeza ukuhlukahluka emhlabeni wezitshalo.

Isiphetho

Sengiphetha, amasu okusabalalisa isiko lezicubu aguqule indlela esitshala ngayo izitshalo zePhilodendron. Ikhono lokukhiqiza ngobuningi izitshalo ezingenazo izifo, ezifana nofuzo ngesikhathi esifushane lilethe izinzuzo eziningi kubalimi bezentengiselwano nabathandi bezitshalo. Isiko lezicubu I-Philodendron ayiqinisekisi nje kuphela ukulondolozwa kwezinhlobo ezingavamile kodwa futhi yenza kube lula ukuhlola izinhlobo ezintsha nokugcinwa kokufana emakethe yezitshalo zamahlamvu. Ngakho-ke, noma ngabe ungumthandi wezitshalo wesikhathi eside noma umlimi osaqala, isiko lezicubu i-Philodendron ngokungangabazeki iyindawo okufanele uyihlole ukuze uthuthukise ihlathi lakho lasendlini.