I-Mastering Tissue Culture I-Philodendron Propagation Techniques

Izingeniso:

Ingabe ungumlimi oshisekayo ofuna ukwandisa iqoqo lakho lezitshalo ze-Philodendron? Noma mhlawumbe ungumuntu othanda isitshalo ofuna ukungena enkingeni yokusakazwa kwesiko lezicubu? Ungabheki okungaphezu kokwazi amasu okusabalalisa izicubu ze-Philodendron! Kulo mhlahlandlela obanzi, sizongena emhlabeni wesiko lezicubu futhi sihlole amasu ahlukahlukene ongawasebenzisa ukuze usakaze ngempumelelo izitshalo zakho ezizithandayo ze-Philodendron. Ngakho-ke, bamba ijazi lakho laselabhu futhi ulungele ukuqala uhambo olujabulisayo endaweni yokusabalalisa izitshalo!

Izisekelo ZeSiko Lwezicubu

I-tissue culture, eyaziwa nangokuthi i-micropropagation, iyindlela ebalulekile yokusabalalisa izitshalo ngaphansi kwezimo eziyinyumba. Kubandakanya ukutshala izingcezu ezincane zezicubu zezitshalo endaweni elawulwayo ukuze kukhule ukukhula nokuthuthuka kwazo zibe izitshalo eziphelele. I-tissue culture ingaba wusizo ikakhulukazi ekusakazeni kwe-Philodendron njengoba ivumela ukukhiqizwa ngokushesha kwezitshalo eziningi ezifana nofuzo, ngisho nasesitshalweni esisodwa. Le nqubo ayisetshenziswa abalimi bezentengiselwano kuphela kodwa nabathandi bezitshalo abafisa ukwandisa iqoqo labo noma ukulondoloza izimila zePhilodendron eziyivelakancane nezibalulekile.

Ukuze uqalise nge-tissue culture Philodendron propagation, kunezinto ezimbalwa ezibalulekile kanye namasu okufanele uzijwayele. Ake sizihlole ngokuningiliziwe.

Ukukhetha Impahla Yezitshalo Efanelekayo

Ukukhetha isitshalo esifanele kubalulekile ekusakazeni ngempumelelo isiko lezicubu. Ngokufanelekile, khetha izibonelo ze-Philodendron ezinempilo ezingenazo izifo nezinambuzane. Khetha izitshalo ezikhula ngokukhuthele ngamathiphu amahlumela amancane noma ama-axillary buds, njengoba zinamandla aphezulu okuvuselela. Kunconywa ukuthi ugweme ukusebenzisa izicubu ezivuthiwe noma ezinokhuni njengoba zingasabeli kakhulu kumasu osiko lwezicubu.

Uma usukhethile isitshalo se-Philodendron osithandayo, qiniseka ukuthi uhlanza isitshalo kahle. Qala ngokugeza izingxenye zesitshalo ngaphansi kwamanzi agobhozayo ukuze ususe noma yikuphi ukungcola noma imfucumfucu. Okulandelayo, cwilisa impahla yesitshalo esitsheni esifanelekile sokubulala amagciwane, njengesixazululo se-bleach esingu-10%, isikhathi esifushane (cishe imizuzu eyi-10). Lesi sinyathelo sisiza ukuqeda noma yikuphi ukungcola okungaphezulu futhi sinciphise ubungozi bokungenisa amagciwane endaweni yesiko lezicubu.

Ngemuva kokubulala amagciwane, hlambulula isitshalo izikhathi eziningi ngamanzi acwengekile angenalutho ukuze ususe iminonjana yesisombululo sesibulala-magciwane. Kubalulekile ukugcina indawo eyinyumba ngokuphelele kuyo yonke le nqubo ukuze kuvinjelwe ukungcoliswa okungafunwa.

Ukwenza I-Culture Medium

I-culture medium iyingxenye ebalulekile yokusabalalisa isiko lezicubu njengoba ihlinzeka ngemisoco edingekayo kanye nezilawuli zokukhula zokuthuthukiswa kwezicubu zezitshalo. Kunezinhlobo ezahlukene zemidiya yesiko etholakalayo, kodwa esetshenziswa kakhulu isiko lezicubu ze-Philodendron i-Murashige ne-Skoog (MS) medium.

Ukuze ulungise isiko, uzodinga ukuhlanganisa usawoti wezinto ezingaphili, izinhlanganisela eziphilayo (njengamavithamini), umthombo wekhabhoni (imvamisa i-sucrose), nezilawuli zokukhula (njengama-auxins nama-cytokinins). Lezi zithako kufanele zixutshwe ngezilinganiso ezithile futhi zihlakazwe emanzini a-distilled. I-pH yendawo ephakathi kufanele ilungiswe ibe cishe ku-5.8 ngaphambi kokuvala inzalo.

Ukuqinisekisa ubunyumba, i-culture medium idinga ukufakwa ngokuzenzakalelayo ngomfutho ophezulu kanye nezinga lokushisa. I-Autoclaving iqeda noma yikuphi ukungcola okungenzeka, okwenza okuphakathi kuhambisane nesiko lezicubu. Uma sekufakwe i-autoclaved, okuphakathi kungathelwa ezitsheni ezingenalutho, njengezitsha ze-Petri noma amashubhu okuhlola, futhi kuvunyelwe ukuthi kuqine. Imikhumbi ye-culture kufanele ibhalwe kahle futhi igcinwe endaweni epholile futhi emnyama kuze kube yilapho isilungele ukusetshenziswa.

Ukusungula Izimo ze-Aseptic

Ukugcina izimo ze-aseptic kubaluleke kakhulu kuyo yonke inqubo yesiko lezicubu ukuvikela ukungcoliswa nokuqinisekisa ukusakazeka ngempumelelo. Njengomuntu oqalayo, kunconywa ukusetha indawo yokusebenza eyinyumba, evame ukubizwa ngokuthi i-laminar flow hood noma i-cleanroom, lapho ungakwazi ukuphatha izinto zezitshalo futhi wenze izinqubo ezidingekayo.

I-laminar flow hood ihlinzeka ngokugeleza okuqhubekayo komoya ohlungiwe kakhulu okunciphisa ubungozi bokwethula ukungcola okuphuma emoyeni emikhunjini yesiko. Ifakwe negumbi lokuvala inzalo lapho ungabeka khona amathuluzi adingekayo, njengama-scalpels, ama-forceps, nemikhumbi yamasiko, ukuze uqinisekise ukuthi ayinyumba ngaphambi kokusetshenziswa.

Ngaphambi kokungena endaweni yokusebenza eyinyumba, kubalulekile ukuthi unikele ngegiya elifanele lokuvikela, okuhlanganisa ijazi laselabhu, amagilavu, kanye nemaski yobuso. Lezi zindlela zokuphepha zinciphisa amathuba okungcolisa impahla yezitshalo kanye nendlela yesiko.

Ukuqala Amasiko Okukhipha

Amasiko e-explant asho izicubu zezitshalo ezibekwe endaweni yesiko ukuze kuqalwe inqubo yesiko lezicubu. Izitshalo zingatholakala ezingxenyeni ezihlukahlukene zesitshalo se-Philodendron, njengamathiphu wokuhluma, ama-node, noma izingxenye zamaqabunga.

Ukuqala isiko lokutshala, qala ngokusika ucezu oluncane lwethishu yesitshalo osifunayo usebenzisa i-scalpel esizinzayo noma i-razor blade. Qinisekisa ukuthi insimbi yokusika ivutha isikhashana futhi ivunyelwe ukuba iphole ngaphambi kokusikwa ngakunye, ukuze kugcinwe ubunyumba. Beka i-explant endaweni eqinile ye-culture medium, uqinisekise ukuthi ithintana ngqo nendawo ukuze kube lula ukukhula.

Uma izinto ezifakiwe sezibekwe endaweni yezamasiko, vala imikhumbi yezamasiko usebenzisa into ephefumulayo, njengeParafilm noma ifilimu yaselabhorethri. Lokhu kuvumela ukushintshaniswa kwegesi ngenkathi kuvimbela ukungcoliswa okuvela emithonjeni yangaphandle. Faka ilebula umkhumbi ngamunye we-culture nosuku lokuqalwa kanye nanoma yiluphi olunye ulwazi olubalulekile ukuze ulandelele ukuqhubeka kwesiko lezicubu.

Isifinyezo

Sengiphetha, amasu okusabalalisa i-Philodendron anika ithuba elijabulisayo lokusabalalisa izitshalo zakho eziyizintandokazi ze-Philodendron ngezinga elikhulu. Ngokufunda kahle izisekelo zesiko lezicubu, okubandakanya ukukhetha isitshalo esifanele, ukulungisa indawo yesiko, ukugcina izimo ze-aseptic, nokuqala amasiko okufakelwa, ungathola impumelelo emangalisayo ekusakazeni ama-Philodendron. Khumbula, ukubekezela nokunaka imininingwane kuyisihluthulelo sokuqinisekisa impumelelo yemizamo yakho yesiko lezicubu. Ngakho-ke, gqoka ijazi lakho laselabhu futhi uzicwilise emhlabeni othakazelisayo wokusakazwa kwezicubu ze-Philodendron!