Ikusasa Le-Tissue Culture Philodendron: Intuthuko Nentuthuko

Isingeniso:

Umhlaba wokutshalwa kwezitshalo ubone intuthuko enkulu eminyakeni yamuva, ngenxa yokwethulwa kwamasu okwenziwa kwezicubu. Isiko lezicubu liye laguqula ukusakazeka kwezinhlobo zezitshalo ezihlukahlukene, kuhlanganise nePhilodendron ethandekayo. Lesi sihloko sidingida intuthuko nentuthuko ethokozisayo kusikompilo lwezicubu i-Philodendron, enikeza imininingwane ngekusasa lalesi sitshalo esimangalisayo. Kusukela ezindleleni zokuzalanisa ezithuthukisiwe kuya ekusakazweni okungenazifo, amathuba abonakala engapheli.

Intuthuko 1: Amasu Okuzalanisa Athuthukisiwe

Ukuzalanisa kudlala indima ebalulekile ekuthuthukiseni izinhlobo ze-Philodendron ezinoveli ezinezici nezici ezihlukile. Isiko lezicubu livule izindlela ezintsha zokuthuthukisa izindlela zokuzalanisa kanye nokuzuza imiphumela efiselekayo. Ngokusebenzisa isiko lezicubu, abafuyi bangakwazi ukudlula imikhawulo evamile ebekwe ukukhiqizwa ngokocansi futhi benze ama-hybrids okungenzeka angenzeki ngezindlela zemvelo.

Ngokusebenzisa isiko lezicubu, abafuyi bangasebenzisa izakhi zofuzo zezitshalo, benze izinguquko futhi baguqule ukwakheka kofuzo kwezinhlobo ze-Philodendron. Lokhu kuvumela ukuthuthukiswa kwezitshalo ezinezimfanelo ezithuthukisiwe, njengokuhlukahluka kombala okuthuthukisiwe, amaphethini ayingqayizivele, nokwanda kokumelana nokucindezeleka. Ngaphezu kwalokho, isiko lezicubu lenza abalimi bakwazi ukukhiqiza izitshalo ezinemikhuba yokukhula ethuthukisiwe, ama-internode amafushane, namafomu aminyene, okuzenza zilungele ukutshalwa endlini.

Intuthuko 2: Ukusakazeka Ngaphandle Kwezifo

Izifo zezitshalo zibeka izinselelo ezinkulu ezindleleni zendabuko zokusabalalisa futhi zingavimbela ukukhula nokuthuthukiswa kwe-Philodendron. Kodwa-ke, isiko lezicubu linikeza isisombululo esisha kule nkinga - ukusakazeka ngaphandle kwezifo. Ngokuqala ngezicubu zezitshalo eziyinyumba, isiko lezicubu lisusa ingozi yamagciwane nezifo, liqinisekisa ukukhiqizwa kwezitshalo ezinempilo nezinamandla.

Emasikweni ezicubu, izicubu zezitshalo, njengamathiphu okudubula noma izingxenye ze-nodal, zihlukanisiwe futhi zikhuliswe ezimeni ze-aseptic. Lokhu kuvumela ukukhula kwezitshalo ezingenalo i-pathogen, kunciphise isidingo sezibulala-zinambuzane futhi kunciphise ingozi yokudluliselwa kwezifo. Ukusakazeka ngaphandle kwezifo akugcini nje ngokuqinisekisa izitshalo ezinempilo kodwa futhi kufaka isandla ekulondolozweni kwezinhlobo ze-Philodendron ezingavamile nezisengozini yokushabalala.

Intuthuko 3: Ukuphindaphindeka Kwezitshalo Ngokushesha

Esinye sezici eziphawuleka kakhulu zesiko lezicubu yikhono layo lokuphindaphinda ngokushesha izitshalo ngesilinganiso esikhulu. Ngenqubo ebizwa ngokuthi i-micropropagation, isiko lezicubu lenza ukukhiqizwa kwezinkulungwane zezitshalo ze-Philodendron kusuka esiqeshini esincane sethishu. Lokhu kusheshisa kokuphindaphindeka kwezitshalo kuguqule ukutholakala kwezentengiso kwezinhlobo ze-Philodendron, okuzenze zifinyeleleke kalula kubathandi bezitshalo emhlabeni wonke.

Ku-micropropagation, izicubu zezitshalo zihlukaniswa zibe izitsha ezincane, ngasinye sinamandla okuthuthuka sibe isitshalo esiphelele. Lezi zitshalo zifakwa emithonjeni yamasiko equkethe imisoco ebalulekile, izilawuli zokukhula, namahomoni, akhuthaza ukukhula nokuthuthuka kwazo. Njengoba izitshalo zanda, zingadluliselwa emikhunjini emikhulu futhi ekugcineni zijwayele indawo yemvelo. Ukukhula okusheshayo kanye nezinga lokuphindaphinda elinikezwa isiko lezicubu liqinisekisa ukutholakala njalo kwezitshalo ze-Philodendron ngezinhloso ezihlukahlukene.

Intuthuko 4: Ukulondolozwa Kwezakhi Zofuzo

Ukulondolozwa kwezinhlobonhlobo zofuzo zezitshalo kuyisici esibalulekile ekuvikeleni ikusasa lezinhlobo ze-Philodendron. I-tissue Culture ayinikezi nje kuphela indlela yokulondoloza izinto zofuzo kodwa futhi inikeza indlela yokubuyisela izilwane ezisengozini ezindaweni zazo zemvelo. Le ntuthuko idlala indima ebalulekile emizamweni yokongiwa kwemvelo, iqinisekisa ukusinda nokukhula kwezinhlobo ze-Philodendron ezingavamile nezisengozini.

Besebenzisa amasu amasiko ezicubu, abacwaningi bangagcina ngempumelelo izakhi zofuzo zezitshalo ngendlela yamaseli e-cryopreserved. Lawa maseli agcinwa emazingeni okushisa aphansi kakhulu, okuqinisekisa ukusebenza kwawo kwesikhathi eside. Ikhono lokulondoloza kanye nempahla yofuzo yasebhange iqinisekisa ukuthi izinhlobonhlobo zezitshalo zivikelekile ezinhlekeleleni zemvelo, izinambuzane, izifo, nokucekelwa phansi kwezindawo zokuhlala. Uma kwenzeka kuba nesidingo, amaseli agcinwe e-cryopreserved angaphindwa kabusha futhi aphinde afakwe ohlelweni lwendalo lwemvelo, okukhuthaze ukutholakala kwezinhlobo ze-Philodendron ezisengozini.

Intuthuko yesi-5: Ukuthuthukiswa Kokumelana Nezifo

Izifo zezitshalo zingacekela phansi izitshalo ze-Philodendron, okuholela ekulahlekelweni okukhulu kwezomnotho kubalimi. I-tissue culture inikeza inkundla yokuthuthukisa ukumelana nezifo e-Philodendron ngokusebenzisa amasu ahlukahlukene. Ngokukhetha kanye nokusabalalisa abantu abamelana nezifo, isiko lezicubu livumela ukukhiqizwa kwezitshalo ezingamelana nezinhlobonhlobo zamagciwane kanye nezinambuzane, kuqinisekiswe isivuno esiphezulu sezitshalo kanye nempilo yezitshalo iyonke.

Ngaphezu kwalokho, isiko lezicubu linikeza ithuba lokusebenzisa amasu wobunjiniyela bezakhi zofuzo ukwethula izakhi zofuzo kuPhilodendron. Lezi zakhi zofuzo zinganikeza ukumelana nezifo ezithile, zisize ukulwa nokuqubuka nokunciphisa ukuthembela ekulawuleni amakhemikhali. Ngokusebenzisa isiko lezicubu, abacwaningi bangahlola futhi bakhethe izitshalo ezinezimpawu ezithuthukisiwe zokumelana nezifo, zinikeze isisombululo esiphumelelayo nesimeme ekulawuleni izifo ekutshalweni kwePhilodendron.

Isiphetho:

Ikusasa lezicubu zesiko I-Philodendron liqhakazile futhi ligcwele amathuba amakhulu. Ukuthuthuka kwezindlela zokuzalanisa, ukusakazeka okungenazifo, ukuphindaphindeka kwezitshalo ngokushesha, ukulondolozwa kofuzo, kanye nokuthuthukiswa kokumelana nezifo kubeke isiko lezicubu njengento eshintsha umdlalo embonini yezolimo. Kusukela ekudaleni izinhlobo eziyingqayizivele kuye ekulondolozeni izinhlobo ezisengozini yokushabalala, isiko lezicubu linikeza amandla amangalisayo okukhula nokuthuthukiswa kwePhilodendron. Njengoba siqhubeka nokuhlola amathuba, kuqinisekile ukuthi isiko lezicubu lizodlala indima ebalulekile ekulolongeni ikusasa lalesi sitshalo esithandekayo.