I-Science Behind Tissue Culture Monstera: Yenziwa Kanjani

Wake wazibuza ukuthi izitshalo zisakazwa kanjani ngezinga elikhulu? I-tissue culture, noma i-micropropagation, ingenye yezindlela ezisetshenziswa embonini ye-horticultural ukukhiqiza inani elikhulu lama-clone ezitshalo kusuka esiqeshini esincane sethishu. Isitshalo seMonstera, esinamaqabunga aso ahlukanisiwe amangalisayo, siyisinqumo esidumile phakathi kwabathandi bezitshalo. Kulesi sihloko, sizocubungula isayensi yesiko lezicubu iMonstera nokuthi lenziwa kanjani.

Izisekelo ZeSiko Lwezicubu

Isiko lezicubu kuyindlela lapho izingcezu ezincane zezicubu zesitshalo, njengesiqu noma amaqabunga, zifakwa endaweni enomsoco ukuze zikhule zibe isitshalo sonke. Le nqubo ivumela ukukhiqizwa kwezitshalo eziningi ezifanayo ngokofuzo ngesikhathi esifushane. Isinyathelo sokuqala esifundweni sezicubu ukukhethwa kwesampula yethishu enempilo esitshalweni esingumzali. Lesi sampula sethishu sibe sesivalwa amagciwane ukuze kukhishwe noma yikuphi ukungcola okungase kuthiye ukukhula. Uma isicubu sesifakwe inzalo, sifakwa endaweni yokukhula equkethe izakhamzimba ezibalulekile, amahomoni, noshukela ukuze kuthuthukiswe ukukhula nokuthuthuka.

Enye yezinzuzo eziyinhloko zesiko lezicubu yikhono lokukhiqiza inani elikhulu lezitshalo endaweni encane. Lokhu kuwusizo ikakhulukazi ezitshalweni ezifana neMonstera, ezinesidingo esikhulu emakethe. Isiko lezicubu futhi livumela ukukhiqizwa kwezitshalo ezingenazo izifo, njengoba inqubo ihilela izindlela eziqinile zokuvala inzalo ukuvimbela ukungcoliswa.

Ukuqaliswa kwe-Tissue Culture Monstera

Isinyathelo sokuqala kusiko lwezicubu i-Monstera ukuqaliswa kwesiko kusuka kusampula yezicubu ezikhethiwe. Lesi sampula sethishu ngokuvamile sithathwa esihlokweni sesitshalo, njengoba siqukethe amangqamuzana e-meristematic anekhono lokuhlukanisa futhi ahlukanise abe izicubu zezitshalo ezahlukene. Ithiphu yokushutha inzalo kusetshenziswa inhlanganisela ye-bleach notshwala ukuze kususwe noma yikuphi ukungcola okungaphezulu.

Uma ithiphu lehlumela selivalwe inzalo, lifakwa endaweni yokukhula equkethe izakhamzimba ezibalulekile, amahomoni, noshukela. Indawo yokukhula ihlinzeka ngemisoco edingekayo ukuze ithiphu lehlumela likhule futhi likhule libe yisitshalo sonke. Ithiphu lehlumela libe selifakwa egumbini lokukhula elinezinga lokushisa elilawulwayo, ukukhanya, kanye nomswakama ukuze kuthuthukiswe ukukhula.

Isiteji Sokwanda

Ngemuva kwesigaba sokuqala, ithiphu lehlumela liqala ukwanda, lenze amahlumela amaningi asuka kusampula yethishu yokuqala. Lesi sigaba sibalulekile ekukhiqizeni inani elikhulu lama-clone ezitshalo. Ukukhuthaza ukwanda, amathiphu wokuthwebula adluliselwa endaweni entsha yokukhula equkethe ukugxila okuphezulu kwamahomoni afana nama-cytokinins, akhuthaza ukuhlukana kwamaseli.

Phakathi nesigaba sokwanda, amahlumela ahlala e-subcultured ukuze alondoloze ukukhula kwawo futhi avimbele ukuminyana. I-Subculturing ihlanganisa ukudlulisa amahlumela endaweni entsha yokukhula ukuze abanike imisoco entsha namahomoni. Le nqubo ivumela ukukhiqizwa okuqhubekayo kwamahlumela amasha kulawo akhona.

Isiteji sezimpande

Uma amahlumela esewandile, asuke eselungele ukugxilwa ukuze akhe isitshalo sonke. Isigaba sokuhluma sibalulekile ukuze kuthuthukiswe uhlelo lwezimpande olunempilo, olubalulekile ekukhuleni nasekuphileni kwesitshalo. Amahlumela adluliselwa endaweni yokukhula equkethe amahomoni afana nama-auxins, akhuthaza ukukhula kwezimpande.

Esigabeni se-rooting, amahlumela afakwa kwisisombululo se-hormone yezimpande ukuze akhuthaze ukukhula kwezimpande. Amahlumela abe esedluliselwa endaweni yokufaka izimpande equkethe izakhamzimba ezibalulekile namahomoni ukuze kuthuthukiswe izimpande. I-rooting medium inikeza izimo ezidingekayo ukuze amahlumela akhe uhlelo lwezimpande oluqinile.

Ukujwayela kanye Nokudluliselwa Emhlabathini

Ngemva kokuba amahlumela esenezimpande, alungele ukujwayela indawo yemvelo ngaphambi kokuba adluliselwe emhlabathini. Ukuzijwayeza kuhilela ukudalula kancane kancane izitshalo zezitshalo emazingeni omswakama ephansi kanye nokukhanya kwemvelo ukuzilungiselela ukuphila ngaphandle kwegumbi lokukhula. Le nqubo isiza izitshalo ukuthi zivumelane noshintsho endaweni futhi zivikele ukushaqeka kokufakelwa.

Uma izitshalo sezijwayele ukujwayela, zidluliselwa emhlabathini ukuze ziqhubeke nokukhula nokukhula kwazo. Umhlabathi kufanele ukhiphe kahle futhi ucebile ngemisoco ukuze usekele ukukhula kwesitshalo. Ukunisela njalo nokuvundiswa kubalulekile ekukhuleni okunempilo kwesiko lezicubu ze-Monstera.

Sengiphetha, isiko lezicubu kuyindlela enamandla yokukhiqiza izitshalo eziningi njengeMonstera. Ngokulandela uchungechunge lwezinyathelo, kusukela ekuqalisweni kuya ekujwayelekeni, kungenzeka ukukhiqiza inani elikhulu lezitshalo ezifanayo zofuzo nezingenazifo ngesikhathi esifushane. Isiko lezicubu Izitshalo zeMonstera zinamandla okuhlangabezana nesidingo esikhulu emakethe ngenkathi zigcina ikhwalithi nezici zesitshalo esingumzali. Ngokudalulwa kwesayensi yesiko lezicubu iMonstera, kuyamangaza ukubona ukuthi le ndlela ilolonga kanjani ikusasa lokusakazeka kwezitshalo.