Izitshalo Ezincane - Abakhiqizi Nabahlinzeki Bezimboni Ezithengisa Izicubu Esitolo Esitolo Esidayisa Izicubu.
I-Tissue Culture Philodendron: Indlela Yokusakaza Esimeme Nephumelelayo
Indlela Yokusakaza Eqhubekayo Nephumelelayo: I-Tissue Culture Philodendron
Isingeniso:
Ama-Philodendron ayizitshalo zokuhlobisa ezidumile ezaziwa ngamahlamvu azo akhangayo kanye nokunakekelwa kalula. Zilinywa kabanzi ngenxa yokukhanga kwazo kobuhle, okuzenza zibe intandokazi phakathi kwabathandi bezitshalo zasendlini. Izindlela zendabuko zokusabalalisa, njengokusikwa kwesiqu noma ukuhluma kwembewu, zivame ukusetshenziselwa ukusabalalisa izinhlobo ze-Philodendron. Nokho, lawa masu avamile anemikhawulo yawo, okuhlanganisa amanani aphansi empumelelo nemiphumela engaguquki. Ukuze kunqotshwe lezi zinselele, isiko lezicubu liye lavela njengendlela eqhubekayo nephumelelayo yokusakaza emhlabeni wokutshalwa kwezitshalo. Kulesi sihloko, sizohlola izinzuzo kanye nobunkimbinkimbi be-tissue culture Philodendron propagation, eqokomisa amandla ayo okuguqula imboni.
Isayensi Engemuva KweSiko Lezicubu
Inqubo yezicubu, eyaziwa nangokuthi i-micropropagation, ihilela ukukhula kwamaseli ezitshalo, izicubu, noma izitho zaselabhorethri ngaphansi kwezimo eziyinyumba. Le nqubo isebenzisa amathuluzi akhethekile ahlukahlukene kanye nemidiya yokukhula ukuze ikhuthaze ukukhula nokuthuthukiswa kwezitshalo. Endabeni ye-Philodendron, isiko lezicubu linikeza ithuba eliyingqayizivele lokusabalalisa izitshalo eziningi ezivela emthonjeni wesitshalo esisodwa, ukuqinisekisa ukufana kwezakhi zofuzo nokukhiqiza izibonelo ezinempilo nezingenazifo.
Inqubo yezicubu iqala ngokukhethwa kwezicubu zesitshalo ezifanele, ngokuvamile i-meristem noma ihlumela le-apical. Ithishu ekhethiwe ibe isivalwa inzalo ukuze kuqedwe noma yikuphi ukungcola kwangaphandle, kulandelwa ukwethulwa endaweni yosikompilo ehlanganisa izakhamzimba ezibalulekile, amavithamini, nezilawuli zokukhula kwezitshalo. Lezi zici zokukhula zikhuthaza ukuhlukana kwamangqamuzana, ukunwetshwa, nokuhlukaniswa kwe-vitro, okuvumela izicubu ukuba zikhule zibe isitshalo sonke. Le nqubo yenzeka endaweni elawulwayo, njengegumbi lokukhula noma i-incubator, lapho izinga lokushisa, umswakama, nezimo zokukhanyisa zigcinwa ngokucophelela.
Izinzuzo Zokusakazwa Kwesiko Kwezicubu
Ukusakazwa kwesiko lezicubu sekuthole ukuqashelwa okubalulekile embonini yezolimo ngenxa yezinzuzo zayo eziningi kunezindlela zendabuko. Ake sihlole ezinye izinzuzo ezibalulekile:
Inqubo ye-Tissue Culture ye-Philodendron
Ukusakazwa kwesiko lezicubu ze-Philodendron kunezigaba eziningana ezihlukene, ngasinye sibalulekile ekukhuleni ngempumelelo nasekuthuthukeni kwezitshalo. Ake sihlole inqubo enemininingwane:
Lesi sinyathelo sokuqala sibandakanya ukukhethwa kwesitshalo esifanelekayo (izicubu ezizotshalwa) esibonelweni sePhilodendron esinempilo nesingenazifo. I-explants evame ukusetshenziswa kakhulu yesiko lezicubu ze-Philodendron yi-meristem noma amathiphu okudubula. Izinto ezikhishwayo zibe sezihlakazwa ngokucophelela futhi zifakwe amagciwane ukuze kukhishwe noma yikuphi ukungcola kwangaphandle noma amagciwane. Uma isivalwe inzalo, izinto ezifakwayo zifakwa endaweni yesiko eliqukethe amahomoni ezitshalo, amavithamini, kanye nezakhamzimba ukuze kugqugquzelwe ukuqala kwamahlumela.
Ngemva kokuqaliswa ngempumelelo, amahlumela avela ezitshalweni adluliselwa endaweni entsha yesiko elungiselelwe ukwakheka nokuphindaphinda kwamahlumela. Lesi sikhungo ngokuvamile sihlanganisa ama-cytokinins, akhuthaza ukwanda kwamahlumela, okuvumela ukuthuthukiswa kwamahlumela amaningi avela esitsheni esisodwa. I-subculturing evamile iyenziwa ukuze kudluliselwe amahlumela asanda kwakheka emithonjeni emisha ukuze kusekelwe ukuphindaphindeka kwawo okuqhubekayo.
Uma isibalo esanele samahlumela sesitholiwe, adluliselwa endaweni yokufaka izimpande ekhuthaza ukukhula kwezimpande. Indawo yokufaka izimpande ivamise ukunothiswa ngama-auxins, afana ne-indole-3-butyric acid (IBA) noma i-naphthalene acetic acid (NAA), ukuze kugqugquzelwe ukuqaliswa nokukhula kwezimpande. Ukuba khona kwamavithamini kanye nezakhamzimba phakathi nendawo kusekela ngokwengeziwe ukukhula kwezimpande.
Uma sezigxilile, ama-plantlets asuswa ngokucophelela emikhunjini yesiko futhi ajwayele izimo ze-ex vitro. Lolu shintsho lwenzeka kancane kancane ukuvumela izitshalo ukuthi zivumelane nezinguquko zemvelo. Ekuqaleni, ama-plantlets agcinwa endaweni elawulwayo enamazinga aphezulu omswakama, kancane kancane anciphise umswakama ngokuhamba kwesikhathi. Ekugcineni, zidluliselwa ezimweni ze-greenhouse noma zifakwe ebhodweni ukuze zidayiswe noma zikhule.
Izinselelo Ekusakazeni Kwezicubu ze-Philodendron
Nakuba ukusakazwa kwesiko lezicubu kunikeza izinzuzo eziningi, kunezinselele zakho. Ukuhlakulela i-Philodendron ngokusebenzisa isiko lezicubu kudinga inani elikhulu lamakhono, ukunemba, kanye nemishini ekhethekile. Ezinye zezithiyo ezivamile okubhekana nazo ngesikhathi senqubo zihlanganisa:
Isifinyezo:
Ukusakazwa kwesiko lezicubu kuguqule izindlela zokusatshalaliswa kwezinhlobo ze-Philodendron, ngendlela eqhubekayo nephumelelayo. Ngokukwazi ukukhiqiza izitshalo ngofuzo ngobuningi unyaka wonke, isiko lezicubu linikeza ukuhlinzekwa okungaguquki kwezibonelo ze-Philodendron ezinempilo nezingenazifo. Nakuba iveza isethi yayo yezinselelo, isiko lezicubu lifakazele ukuthi lishintsha umdlalo embonini yezolimo. Njengoba isidingo se-Philodendron siqhubeka sikhuphuka, isiko lezicubu linikeza indlela ethembisayo yokuhlangabezana nalesi sidingo nokuqinisekisa ukutholakala kwalezi zitshalo ezimangalisayo eminyakeni ezayo.
. I-Foshan Yangplants ingumhlinzeki wezitshalo zamasiko wezicubu ezithengiswayo kanye nomkhiqizi e-China ngeminyaka eminingi. AmaYangplants ahlinzeka ngezinhlobonhlobo zePhilodendron, Alocasia, Caladium, Aglaonema, Diefffenbachia, Spathiphyllum, Calathea, Fern, Fittonia, Syngonium, Peperomia, Izitshalo Ezidliwayo, Dracaena, Ficus, kanye neSchefflera. I-Foshan Youngplants inethemba lobuqotho lokusebenza nabalimi, izinkulisa, amapulazi, abalimi, kanye namalebhu ukwethula nokuhlinzeka ngezilimo ezintsha ezengeziwe kubantu emhlabeni jikelele.