Amasu Wesiko Lezicubu Wokufeza Ukukhula kwe-Anthurium

Ukuvula Amandla Ezindlela Zesiko Lezicubu Zokukhula kwe-Anthurium

I-Anthirium isitshalo esiqhakazayo esimangalisayo nesidumile esengeza ukuthinta kobuhle kunoma iyiphi ingadi noma indawo yasendlini. Kodwa-ke, ukufeza ukukhula okuhle nokuqhakaza kwe-Anthuriums kwesinye isikhathi kungaba yinselelo, ikakhulukazi kwabaqalayo. Izindlela ezivamile zokusakaza, njengembewu noma ukusikwa, zingase zingavezi imiphumela engcono kakhulu ngaso sonke isikhathi. Yilapho kungena khona amasu amasiko ezicubu. I-tissue culture iyindlela esezingeni eliphezulu evumela ukusabalala ngokushesha kwezitshalo ngaphansi kwezimo ezilawulwayo, okuholela emifanekisweni enempilo futhi eqinile. Kulesi sihloko, sizohlola amasu ahlukahlukene we-tissue culture angasiza ukukhula okuphelele kwe-Anthurium, ukuqinisekisa ukuthi ungakwazi ukujabulela lezi zitshalo ezinhle ngokusemandleni azo aphelele.

Izisekelo ZeSiko Lwezicubu

I-tissue culture, eyaziwa nangokuthi i-micropropagation, iyindlela yokutshala izitshalo kusuka ezicutshini zezitshalo ezincane ngaphansi kwezimo eziyinyumba. Le nqubo iye yashintsha indlela izitshalo ezisakazwa ngayo, ivumela ukukhiqizwa ngobuningi kwezitshalo ezinezici ezifiselekayo. Endabeni ye-Anthuriums, isiko lezicubu lingasetshenziswa ukusabalalisa izitshalo ezifana nesitshalo somzali, ukuqinisekisa ukungaguquguquki ezicini ezinjengombala, ubukhulu, namaphethini ezimbali.

Inqubo yesiko lezicubu iqala ngokukhetha isitshalo esinempilo njengomnikeli wezicubu. Izicucu ezincane zezicubu, njengeqabunga noma isiqu, zibe sezithathwa esitshalweni esinikelayo futhi zibekwe endaweni enomsoco equkethe izakhamzimba ezibalulekile, amavithamini, namahomoni ukuze kuthuthukiswe ukukhula. I-medium ilinganiswe ngokucophelela ukuze inikeze imisoco edingekayo ukuze izicubu zikhule zibe isitshalo esisha ngokuphelele. Uma kunezimo ezifanele, izicubu zizokhula zibe isitshalo esincane esingadluliselwa emhlabathini futhi sikhule sibe isitshalo esivuthiwe.

Isiko lezicubu linikeza izinzuzo ezimbalwa kunezindlela zendabuko zokusakaza. Enye yezinzuzo ezibalulekile yikhono lokukhiqiza izitshalo eziningi ngesikhathi esifushane, okuvumela ukwanda ngokushesha kweqoqo lezitshalo noma ukukhiqizwa kwezitshalo ngezinhloso zokuhweba. Ukwengeza, isiko lezicubu liqeda ingozi yezinambuzane nezifo ezingasakazwa ngembewu noma ukusikwa, ukuqinisekisa ukuthi izitshalo ezintsha ziphilile futhi ziqinile.

Ukusungula Ilabhorethri Yamasiko Ezicubu

Ukusetha ilabhorethri yesiko lezicubu kubalulekile ekusakazeni ngempumelelo i-Anthurium ngokusebenzisa isiko lezicubu. Indawo ezinikele enemishini efanele kanye nempahla iyadingeka ukuze kwakhiwe indawo eyinyumba lapho izitshalo zingatshalwa ngaphandle kokungcola. Izingxenye ezibalulekile zelabhorethri yesiko lezicubu zihlanganisa ama-laminar flow hoods, ama-autoclave emishini yokubulala amagciwane, amagumbi okukhula okugcina izinga lokushisa elilungile nezimo zokukhanya, kanye nezinhlobonhlobo zemithombo yezakhi zomzimba zokukhula kwezitshalo.

Ngaphambi kokuthi uqale ukusabalalisa isiko lezicubu, kubalulekile ukuzijwayeza nemigomo yenqubo ye-aseptic ukuvikela ukungcoliswa kwezicubu zesitshalo. Lokhu kuhlanganisa ukufaka amagciwane kuzo zonke izinto zokusebenza nezindawo zokusebenza, kanye nokugqoka izinto ezifanele zokuzivikela, njengamagilavu ​​namajazi elebhu. Ukugcina indawo yokusebenza ihlanzekile futhi ihlelekile kubalulekile empumelelweni yokuhlolwa kwesiko lezicubu.

Uma ilabhorethri ye-tissue Culture isimisiwe, sekuyisikhathi sokulungiselela izicubu zesitshalo ukuze zilungele isiko. Khetha isitshalo se-Anthurium esinempilo njengomnikezeli bese ubulala inzalo yesitshalo ngokuyigeza ngesisombululo sokubulala amagciwane, njenge-bleach noma utshwala. Hlukanisa ngokucophelela izicubu zesitshalo zibe izingcezu ezincane, uqinisekise ukuthi ucezu ngalunye luqukethe inani elanele lamaseli okukhula. Beka izingcezu zezicubu endaweni yezakhi bese uzifukamela ngaphansi kwezimo ezifanele zokukhula.

Ukuthuthukisa Izimo Zokukhula kwe-Anthuriums

Ukudala indawo ekahle yokukhula kwe-Anthurium kubalulekile ekusakazeni isiko lezicubu eziphumelelayo. I-Anthuriums ikhula kahle ezimeni ezifudumele nezinomswakama, ezifana nendawo yazo yendabuko yasezindaweni ezishisayo. Ukugcina izinga lokushisa elingu-70-80��F kanye nomswakama olinganiselwe ongu-70-80% kulungele ukukhuthaza ukukhula nokuthuthukiswa kwe-Anthuriums.

Ukukhanya kungenye into ebalulekile ekukhuleni kwe-Anthurium. I-Anthuriums idinga ukukhanya okukhanyayo, okungaqondile ukuze ichume, njengoba ukukhanya kwelanga kungabangela ukushisa kwamaqabunga futhi kulimaze isitshalo. Ukubeka izitshalo eduze kwefasitela ezinokukhanya kwelanga okuhlungiwe noma ukusebenzisa izibani ezikhulayo kungasiza ukunikeza inani elifanele lokukhanya ukuze zikhule kahle. Ukwengeza, ukuqinisekisa uhlelo lokukhanya olungaguquki lwamahora ayi-12-14 ngosuku kuzosiza ukulawula umjikelezo wokukhula kwesitshalo.

Ngokuphathelene nezidingo zomsoco, i-Anthuriums izuza kumanyolo olinganiselayo ohlinzeka ngezakhi ezibalulekile ezifana ne-nitrogen, i-phosphorus, ne-potassium. Kunconywa umanyolo okhishwa kancane onesilinganiso se-NPK esingu-20-20-20 kuma-Anthuriums, asetshenziswe engxenyeni yamandla anconyiwe njalo emavikini angu-6-8 ngesikhathi sokukhula. Gwema ukuthela umanyolo ngokweqile, ngoba lokhu kungaholela ekubeni ube nobuthi bezakhi futhi kulimaze izimpande zesitshalo.

Ukunisela esinye isici esibalulekile sokunakekelwa kwe-Anthurium. I-Anthuriums ikhetha inhlabathi emanzi njalo, kodwa isengozini yokubola kwezimpande uma inamanzi amaningi. Vumela intshi ephezulu yomhlabathi yome phakathi kokunisela ukuvimbela izimo ezigcwele amanzi ezingaminyanisa izimpande. Ukusebenzisa ingxube yebhodwe edonsa kahle futhi kunikeze umoya omuhle ukuzungeza isitshalo nakho kungasiza ekuvimbeleni ukubola kwezimpande nezinye izifo zesikhunta.

Ukunqoba Izinselele ku-Anthurium Tissue Culture

Ngenkathi isiko lezicubu linganikeza izinzuzo eziningi zokusabalalisa i-Anthuriums, kukhona nezinselelo ezingase ziphakame phakathi nenqubo. Inkinga eyodwa evamile ukungcoliswa kwezicubu zesitshalo ngamagciwane, isikhunta, noma amanye amagciwane. Ukungcola kungenzeka uma inqubo ye-aseptic ingalandelwa kahle noma uma izakhi zomsoco zingenalo inyumba. Ukuze uvimbele ukungcola, kubalulekile ukugcina indawo yokusebenza ihlanzekile neyinyumba, njalo ukhiphe amagciwane amathuluzi, futhi uqaphe amasiko ukuze uthole izimpawu zokungcola.

Enye inselele kusiko lwezicubu ze-Anthurium ukwakheka kwezicubu ze-callus, okuyinqwaba engahlukanisiwe yamangqamuzana angathuthuka ezicubu zesitshalo. Ukwakheka kwe-Callus kungavimbela ukukhula kwezitshalo ezinempilo futhi kuvimbele ukukhula kwezimpande namahlumela. Ukuze unqobe le nselelo, kubalulekile ukwandisa izinga lezakhi zomzimba kanye nama-hormone ukuze kuthuthukiswe ukukhula kwezitshalo ngenkathi kunciphisa ukwakheka kwe-callus. Ukulungisa ukwakheka kokugxila okuphakathi nendawo kanye nama-hormone kungasiza ukukhuthaza ukuthuthukiswa kwezicubu zezitshalo ezinempilo.

Ukuzinza kwezakhi zofuzo kungenye into ekhathazayo esikweni lezicubu ze-Anthurium, njengoba ukuguqulwa kofuzo noma okungavamile kungenzeka ngesikhathi senqubo yokusabalalisa. Ukuqinisekisa ukufana kwezakhi zofuzo nokuzinza, kubalulekile ukukhetha ngokucophelela isitshalo somnikeli esinezici ezifiselekayo futhi usakaze izicubu zezitshalo ngaphansi kwezimo ezilawulwayo. Ukuqapha njalo ama-plantlets ukuthola noma yiziphi izimpawu zokungajwayelekile kwezakhi zofuzo kanye nokukhetha izibonelo ezinempilo kakhulu ukuze ziphinde zisakazeke kungasiza ekugcineni ukusimama kwezakhi zofuzo kubantu bezitshalo.

Ukuthuthukisa Ukukhula kwe-Anthurium ngamasu eSiko Lwezicubu

Sengiphetha, amasu amasiko ezicubu anikeza ithuluzi elibalulekile lokusabalalisa i-Anthuriums nokufeza ukukhula okuhle nokuqhakaza kulezi zitshalo ezinhle. Ngokusungula ilabhorethri yesiko lezicubu, ngokuthuthukisa izimo zokukhula, nokunqoba izinselele enqubweni yokusakaza, ungavula amandla aphelele e-Anthuriums futhi ujabulele iqoqo lezitshalo ezinempilo nezinempilo. I-tissue culture ayivumeli nje kuphela ukukhiqizwa okusheshayo kwezitshalo ezinezici ezifiselekayo kodwa futhi iqinisekisa ukufana kwezakhi zofuzo nokuzinza kwezitshalo. Ngamasu afanele nokunakekela, ungakwazi ukukhula kahle i-Anthurium futhi udale ukuboniswa okumangalisayo kwalezi zimbali ezingavamile ekhaya noma engadini yakho.