Ukuqonda I-Aglaonema Tissue Culture: Izindlela Zokukhiqiza Nokusabalalisa

Isingeniso

I-Aglaonema, evame ukubizwa ngokuthi i-Chinese Evergreens, izitshalo zokuhlobisa ezithandwayo ezithandwa ngamahlamvu azo anemibala nekhono lokuchuma endlini. Lezi zitshalo ezimangalisayo sezifunwa kakhulu, okuholela ekwenyukeni kwesidingo sokukhiqizwa kwezicubu ze-Aglaonema nezindlela zokusabalalisa. Isiko lezicubu linikeza indlela enokwethenjelwa nephumelelayo yokusabalalisa inani elikhulu lezitshalo ezifanayo, ukugcina izici ezifiselekayo zezitshalo zomzali kuyilapho kuqedwa izifo nezinambuzane. Kulesi sihloko, sizongena emhlabeni othakazelisayo we-Aglaonema tissue culture, sihlole izindlela ezihlukahlukene zokukhiqiza nezindlela zokusabalalisa.

Izinzuzo Zesiko Lezicubu ze-Aglaonema

Isiko lezicubu ze-Aglaonema linikeza izinzuzo eziningi ezifaka isandla ekudumeni kwale ndlela. Enye yezinzuzo eziyinhloko ukukhiqizwa kwama-clone ezitshalo ezifanayo. Ngokusebenzisa isiko lamathishu, kuba nokwenzeka ukusabalalisa inani elikhulu lezitshalo ze-Aglaonema ezinofuzo ezifana nesitshalo esingumzali. Lokhu kufana kubaluleke kakhulu uma kuziwa ekukhiqizweni kwezentengiso nokugcina izici ezithile, njengamaphethini okushintshashintsha kwamaqabunga noma umbala.

Ukwengeza, isiko lezicubu linikeza izindlela ezisebenzayo zokuqeda izifo nezinambuzane. Izitshalo ze-Aglaonema zingenwa izifo ezihlukahlukene kanye nokuhlaselwa, okungaphazamisa ukukhula kwazo kanye nempilo yonke. Ngokuqala ngesiqephu esincane sezicubu ezingenazo izifo, isiko lezicubu liqinisekisa ukuthi izitshalo ezisatshalaliswa azinazifo noma izinambuzane ezikhona. Lokhu kuhumusha izitshalo ezinempilo futhi eziqinile, okwandisa inani lazo lemakethe.

Ukulungiselela I-Substrate Yesiko Lezicubu Ze-Aglaonema

Isinyathelo sokuqala kusiko lwezicubu ze-Aglaonema ukulungiswa kwe-substrate efanelekile. Le substrate inikeza imisoco edingekayo, ukwesekwa, kanye nomswakama odingekayo ukuze kukhule kahle izitsha. I-substrate esetshenziswa kakhulu yesiko lezicubu ze-Aglaonema i-Murashige ne-Skoog (MS) medium, ehambisana nezilawuli zokukhula ezifana nama-auxin nama-cytokinins.

Ukulungisa i-substrate, izinyathelo ezilandelayo zilandelwa:

Ukukhethwa kwe-Aglaonema Explants

Ama-explants yizingcezu ezincane zezicubu ezithathwe esitshalweni esingumzali futhi zisetshenziswe njengezinto zokuqala zesiko lezicubu. Ukukhetha izichazi ezinempilo nezingenazifo kubalulekile ukuze kuqinisekiswe ukusakazeka ngempumelelo. Ukusikwa kwamaqabunga, amathiphu okuthwebula, noma izingxenye ze-nodal ngokuvamile zisetshenziswa njenge-Aglaonema explants.

Ukwenziwa inzalo ebusweni

Ukuvala inzalo ebusweni kuyadingeka ukuze kuqedwe noma yimaphi amabhaktheriya, isikhunta, noma okunye ukungcola okukhona endaweni yezinto ezifakiwe. Lokhu kufezwa ngokucwilisa okufakiwe ku-ejenti ebulala amagciwane, njengesixazululo se-bleach noma i-ethanol. Kufanele kuqashelwe ukuthi inqubo yokuvala inzalo ayilimazi noma ilimaze izicubu ezithambile.

Ukulungiswa kwe-MS Medium

I-Murashige ne-Skoog (MS) medium ilungiswa ngokuncibilikisa inani elifanele le-MS basal salts namavithamini emanzini acwecwe. I-pH yendawo ephakathi iyalungiswa, futhi ama-agent aqinisayo afana ne-agar ayengezwa ukuze kuguqulwe uketshezi oluwuketshezi lube ukungaguquguquki okufana nejeli.

Ukwengezwa Kwezilawuli Zokukhula

Izilawuli zokukhula, ezifana nama-auxins nama-cytokinins, zengezwa ku-MS medium ukulawula ukukhula nokuhlukaniswa kwe-explants. Inhlanganisela ethize kanye nokugxilisana kwezilawuli zokukhula kuncike kumphumela oyifunayo, njengokwanda kwamahlumela noma ukwakheka kwezimpande.

Ukutshala kanye ne-Incubation

Uma i-substrate isilungisiwe, izitsha ezifakwe inzalo zibekwe ngokucophelela phezu kwendawo ephakathi. Iziqukathi zibe sezivalwa futhi zibekwe endaweni elawulwayo, ngokuvamile igumbi lokukhula noma i-greenhouse, enokukhanya okwanele nezimo zokushisa. Ama-explants avunyelwe ukukhula futhi akhule abe yizitshalo ezintsha esikhathini esingamaviki ambalwa.

Ukudluliswa kwePlantlet kanye nokujwayela

Ngemuva kokuthi ama-explants athuthukile abe yizitshalo ezinempilo emithanjeni ye-tissue culture, zidinga ukudluliselwa endaweni ekhulayo efanele futhi zijwayele indawo yemvelo. Lesi sinyathelo siqinisekisa ukuguqulwa okuphumelelayo kwezitshalo kusukela ezimeni ezilawulwayo zesiko lezicubu kuya ezimweni ezijwayelekile zokukhula.

Ukudlulisa nokujwayela inqubo ibandakanya lezi zinyathelo ezilandelayo:

Ukususwa Kwezitshalo Ezitshalweni Ezikhungweni

Ama-plantlets asuswa ngokucophelela ezitsheni ze-tissue culture, ngokucophelela ukuthi angonakalisi izimpande ezintekenteke noma amahlumela. I-agar eyengeziwe noma i-gel medium enamathela kuma-plantlets igezwa kahle ngokusebenzisa amanzi acwecwe.

Ukulungiselela Ukukhula Okumaphakathi

Indawo efanelekile yokutshala ilungiselelwa ukusungula ama-plantlets ezimbizeni ezivamile noma ezitsheni. Lesi sikhungo sokukhula kufanele sinikeze ukugeleza okuhle, ukungena komoya, kanye nokutholakala kwezakhi ukuze kusekele ukukhula kwezitshalo okunempilo. Ingxube ye-peat moss, i-perlite, ne-vermiculite ivame ukusetshenziswa njengendawo yokukhula yezitshalo ze-Aglaonema.

Ukutshalwa kabusha kwama-Plantlets

Ama-plantlets atshalwa ngokucophelela emabhodweni ngamanye noma ezitsheni ezigcwele indawo yokukhula elungisiwe. Kufanele kuqashelwe ukuthi izimpande zisabalele futhi aziminyana, okuvumela ukukhula nokuthuthuka okungavinjelwe.

I-Acclimatization ku-Greenhouse Conditions

Izitshalo ze-Aglaonema ezisanda kutshalwa kabusha zifakwa endaweni ebamba ukushisa noma endaweni elawulwa efanayo enezinga lokushisa elikahle kanye namazinga omswakama. Lokhu kusiza izitshalo ukuthi zivumelane nezimo zemvelo kancane kancane. I-shading eyanele kanye nokunisela okuvamile kubalulekile phakathi nenkathi ye-acclimatization ukuvimbela ukucindezeleka nokukhuthaza ukukhula okunempilo.

Dlulisela kuzilungiselelo zangaphakathi noma zangaphandle

Uma ama-plantlets esezijwayele ngempumelelo izimo ze-greenhouse, angadluliselwa ezindaweni zawo zokugcina ezikhulayo, kungaba ngaphakathi noma ngaphandle. Ukunakekelwa nokunakekelwa okufanele, okuhlanganisa ukuchelela njalo, izimo zokukhanyisa ezifanele, nokulawulwa kwezinambuzane, kufanele kuhlinzekwe ukuze kuqinisekiswe ukukhula kwazo okuqhubekayo nokuphila kahle.

Isiphetho

I-Aglaonema tissue culture inikeza indlela ephumelelayo nethembekile yokukhiqiza nokusabalalisa lezi zitshalo zokuhlobisa ezikhangayo. Ukusetshenziswa kwesiko lezicubu kuqinisekisa ukufana kwezakhi zofuzo, izitshalo ezingenazo izifo, kanye nenani lemakethe elithuthukisiwe. Ngokulandela izinyathelo zokulungiswa kwe-substrate, ukukhethwa kokutshalwa kwezitshalo, ukuvala inzalo, nokujwayelanisa, abalimi bangasakaza ngempumelelo inani elikhulu lezitshalo ze-Aglaonema ezinezici ezifiselekayo. Kungakhathaliseki ukuthi okokukhiqiza ukuthengisa noma ukuzijabulisa komuntu siqu, isiko lezicubu linikeza indlela ethembisayo yokwandisa ukutholakala kwezitshalo ze-Aglaonema ukuze zisetshenziswe ngaphakathi nangaphandle.