I-Philodendron TC Plant Guide: Ukusabalalisa Izinhlobo Ezingavamile Ezihlukahlukene Ku-Vitro

Isingeniso:

Ama-Philodendron athandwa kakhulu abathandi bezitshalo ngenxa yamahlamvu awo ahlukile kanye nezidingo zokunakekelwa okulula. Phakathi kwezinhlobo ezihlukahlukene zezitshalo ze-Philodendron, izinhlobo ezingavamile ze-variegated zifunwa kakhulu ngamaphethini azo amangalisayo nemibala. Kulo mhlahlandlela, sizohlola inqubo yokusabalalisa lezi zinhlobo ezingavamile ze-variegated ze-Philodendron in vitro ngokusebenzisa amasu we-tissue culture (TC). Ngokulandela lezi zinyathelo, ungakwazi ukuphindaphinda izitshalo zakho eziyigugu ze-Philodendron futhi wandise iqoqo lakho.

Izinzuzo Zokusakazwa Kwesiko Kwezicubu

Ukusatshalaliswa kwesiko lezicubu kunikeza izinzuzo ezimbalwa zezitshalo ze-Philodendron eziyivelakancane. Enye inzuzo eyinhloko ikhono lokukhiqiza izitshalo eziningi ezifanayo nezingenazifo endaweni elawulwayo. Le ndlela ivumela ukukhiqizwa ngobuningi bezitshalo ezinezici ezifiselekayo, njengamaphethini e-variegation asebenzayo, okuqinisekisa ukufana kokubukeka phakathi kwezibonelo ezisakazwayo. Ukwengeza, ukusakazeka kwesiko lezicubu kungasiza ekulondolozeni nasekulondolozeni izinhlobo ezingavamile ze-variegated ngokunikeza indlela yokusabalalisa izitshalo okungenzeka kube nzima ukuzisakaza ngezindlela zendabuko.

I-tissue culture iphinde ivumele ukuphindaphinda ngokushesha kwezitshalo, okwandisa kakhulu ukusebenza kahle kokusabalalisa uma kuqhathaniswa nezinye izindlela. Lokhu kuzuzisa kakhulu ezitshalweni zePhilodendron eziyivelakancane eziyivelakancane, lapho ukugcinwa kobuqotho besitshalo kubalulekile ukuze kulondolozwe izici zazo ezihlukile. Ngokusebenzisa amasu okwenziwa kwezicubu, ungakwazi ukukhuphula ngokushesha ukukhiqizwa kwakho kwezitshalo ze-Philodendron ezinhlobonhlobo futhi uhlangabezane nesidingo sabaqoqi nabashisekeli abafuna lezi zibonelo eziyigugu.

Ngaphezu kwalokho, ukusakazwa kwesiko lezicubu kunikeza ithuba lokuhlola nokuhlola ama-hybrids amasha noma ukuhluka phakathi kwezinhlobo ze-variegated Philodendron. Ngokusebenzisa izimo zokukhula nama-hormone endaweni ye-tissue culture, abalimi bangakwazi ukuletha izinguquko ku-morphology yezitshalo namaphethini okushintshashintsha, okuholela ekudalweni kwezinhlobo ezintsha zezitshalo. Lokhu kusungulwa kokuzalaniswa kwezitshalo kuvula amathuba ajabulisayo okuthuthukisa izinhlobo ezintsha nezihlukile ze-Philodendron ezingase zihehe abathandi bezitshalo emhlabeni wonke.

Ukumisa ilebhu yesiko lezicubu

Ngaphambi kokuqala ukusabalalisa izinhlobo ze-Philodendron ezingavamile ze-variegated in vitro, kubalulekile ukusungula ilebhu efanele yesiko lezicubu efakwe amathuluzi nezinto zokwakha ezidingekayo. Indawo yokusebenza eyinyumba ibalulekile ukuvimbela ukungcoliswa kanye nokuqinisekisa impumelelo yokusabalalisa isiko lezicubu. Ilebhu kufanele ifakwe ama-laminar flow hoods okusebenza ngaphansi kwezimo ze-aseptic, kanye nama-autoclave emishini yokubulala amagciwane kanye nemidiya. Ngaphezu kwalokho, isikhala esizinikezele esinezibani ezifanele nezilawuli zokushisa zizosiza ukudala izimo ezifanele zokukhula kwezitshalo nokuthuthukiswa kwesiko lezicubu.

Ukumisa ilebhu yesiko lethishu yokusabalalisa izimila ze-Philodendron eziyivelakancane eziyivelakancane, qala ngokuhlanganisa izinto ezibalulekile nezinto ezisetshenziswayo, njengemikhumbi yamasiko, izingxenye zemidiya, kanye nezilawuli zokukhula kwezitshalo. Khetha isikhungo sokukhula esifanele izidingo ezithile ze-Philodendron tissue culture, ehlanganisa imisoco, amavithamini, namahomoni ukusekela ukukhula nokuphindaphindeka kwezitshalo. Qinisekisa ukuthi zonke izinto ezisetshenziswayo ziyavalwa inzalo ngaphambi kokusetshenziswa ukuze kuvinjelwe ukungenwa yisikhunta, amagciwane, noma amanye amagciwane angase abeke engcupheni inqubo yesiko lezicubu.

Gcina inhlanzeko kanye nenhlanzeko elebhu yesiko ngokulandela imithetho eqinile yokuvala inzalo nokuthuthwa kwendle. Sebenzisa amagilavu ​​alahlwayo, imaski yobuso, namajazi elebhu ukuvikela ukungcoliswa ngengozi kwemikhumbi yamasiko nezicubu zezitshalo. Hlanza njalo futhi ubulale amagciwane indawo yokusebenza, amathuluzi, nezinto zokusebenza ukuze unciphise ubungozi bokungenisa ukungcola endaweni yesiko lezicubu. Ngokusungula indawo yokusebenza ehlanzekile neyinyumba, ungakha indawo ekahle yokusabalalisa isiko lezicubu ezingavamile zezinhlobo ze-Philodendron eziyivelakancane.

Ukuqala Isiko Lezicubu Ezivela Ezitshalweni

Isinyathelo sokuqala ekusakazeni izimila zePhilodendron eziyivelakancane eziyivelakancane ku-vitro ukuqala isiko lezicubu ezitholwe esitshalweni sikamama esinempilo nesingenazifo. Khetha ingxenye efanelekile yesitshalo, njengethiphu lehlumela, ingxenye yeqabunga, noma i-node, njengendawo yokutshalwa kokuqaliswa kosiko lwezicubu. Qinisekisa ukuthi ukufakelwa akunazimpawu ezibonakalayo zesifo, ukulimala, noma ukungcoliswa, njengoba lezi zici zingaba nomthelela empumelelweni yokusabalalisa isiko lezicubu.

Lungiselela i-explant ngokuyibulala inzalo usebenzisa uchungechunge lweziwashi ezibulala amagciwane, njenge-bleach noma utshwala, ukususa amagciwane kanye nokungcola. Nciphisa ukufakwa kusayizi nokuma okufunayo, uqinisekise ukuthi kuqukethe izicubu ze-meristematic ezidingekayo zokuqalisa ukukhula kwesiko lethishu. Uma isitshalo sesifakwe amagciwane ngendlela efanele futhi sesilungisiwe, sisuke sesilungele ukwethulwa endaweni yezicubu ukuze kuqalwe ukukhula kwezitshalo.

Khetha isiko elifanelekile lokuqala isiko lezicubu kusukela ekutshalweni, unikeze imisoco edingekayo, izilawuli zokukhula, kanye nezithako ezisekela ukukhula nokuthuthukiswa kwezicubu zesitshalo. Beka isitshalo endaweni yesiko endaweni eyinyumba, uvale umkhumbi wamasiko ukuze ugcine izimo ze-aseptic. Gxilisa umkhumbi wamasiko ngaphansi kwezimo ezilawulwayo zokukhanya, izinga lokushisa, kanye nomswakama ukuze ukhuthaze ukuqaliswa kokukhula kwezitshalo kusuka ku-explant in vitro.

Gada inqubekelaphambili yokuqalwa kwesiko lezicubu kusuka ekutshalweni ngokubheka ukwakheka kwe-callus, ihlumela, nezakhiwo zezimpande esicutshini sesitshalo. I-Subculture izitshalo ezisathuthuka zibe isikhungo esisha samasiko ukukhuthaza ukukhula kwazo okuqhubekayo kanye nokuphindaphindeka kwesiko lezicubu. Ngokuqapha ngokucophelela kanye nokunakekela inqubo yokuqalisa isiko lezicubu, ungasungula isiko lezitshalo elinempilo nelinamandla kusukela ekutshalweni kwezimila zePhilodendron eziyivelakancane eziyivelakancane.

Ukusungula ama-Micropropagation Protocols

Uma ukuqaliswa kwesiko lezicubu kusuka ekufakweni sekuphumelele, isinyathelo esilandelayo ukusungula imigomo ye-micropropagation yokuphindaphinda izinhlobo ze-Philodendron eziyivelakancane eziyivelakancane ku-vitro. Dala inqubo ehlelekile yokukhulisa i-subculturing kanye nokuphindaphinda ama-plantlets atholwe ekuqalisweni kwesiko lezicubu, ukuqinisekisa ukukhula okungaguquki kanye nokuphindaphinda kwezicubu zezitshalo. Ngokulandela amaphrothokholi ajwayelekile okusabalalisa i-micropropagation, ungakwazi ukukhiqiza ngempumelelo inani elikhulu lezitshalo ezinezici ezifanayo zokusabalalisa ngobuningi.

I-Subculture izitshalo ezikhulayo ezikhulayo ngezikhathi ezithile endaweni entsha yamasiko ukuze ukhuthaze ukukhula kwazo okuqhubekayo kanye nokuphindaphindeka kumasiko wezicubu. Gada ukukhula nokukhula kwezitshalo, ulungise izimo zamasiko kanye nezilawuli zokukhula njengoba kudingeka ukuze kuthuthukiswe ukukhula nokuphindaphindeka kwezitshalo. Ngokusungula isimiso esivamile se-subculture kanye nokuqapha impilo namandla ezicubu zesitshalo, ungagcina isiko elinempilo nelikhiqizayo lezinhlobo ezingavamile ze-Philodendron ze-variegated in vitro.

Sebenzisa amasu okwanda kwehlumela, ukwakheka kwezimpande, kanye nokujwayela kwezitshalo ukuze uzilungiselele ukudluliselwa emhlabathini noma ingxube yebhodwe. Thuthukisa amasu okufakwa kwezimpande kanye nokuthuthukiswa kwesiko lezicubu, ukuhlinzeka ngamahomoni adingekayo kanye nezakhamzimba ukukhuthaza ukukhula kwezimpande kuma-plantlets. Yenza ama-plantlets afinyelele izinguquko kancane kancane ezimweni zemvelo, ezifana nokuqina kokukhanya, umswakama, kanye nezinga lokushisa, ukuze azilungiselele ukushintshela okuphumelelayo enhlabathini noma ingxube ye-potting ngemva kokusakazwa kwesiko lezicubu.

Sungula iphrothokholi ye-micropropagation ebanzi yezimila ze-Philodendron eziyivelakancane ezichaza izinyathelo ze-subculture, ukuphindaphindeka, kanye nokujwayelanisa ama-plantlets kusiko lwezicubu. Ngokulandela iphrothokholi ehlelekile kanye nokuqapha inqubekelaphambili yokukhula kwezitshalo esigabeni ngasinye se-micropropagation, ungaqinisekisa ukuphindaphinda okuyimpumelelo kwezitshalo zePhilodendron eziyivelakancane eziyivelakancane ku-vitro. Ngenqubo echazwe kahle ye-micropropagation ekhona, ungasabalalisa ngokuzethemba futhi wandise iqoqo lakho lalezi zimila eziyigugu nezifunwayo ngokusebenzisa amasu osiko lwezicubu.

Ukuthuthukisa Ukulinywa kwePhilodendron Ehlukahlukene

Njengoba uthola ulwazi ekusakazeni izinhlobo ze-Philodendron eziyivelakancane eziyivelakancane ku-vitro, ungahlola amasu namasu athuthukile okuthuthukisa ukutshalwa kwalezi zinhlobo zezitshalo ezihlukile. Zama ukwakheka kwemidiya yamasiko ahlukahlukene, izilawuli zokukhula, nezimo zemvelo ukuze ukhulise ukukhula, ukuhlukahluka, kanye nempilo yonke yezitshalo ze-variegated Philodendron kusiko lamathishu. Ngokulungisa kahle izinqubo namasu okulima, ungafinyelela imiphumela ephezulu ekusakazeni nasekukhuliseni izimila zePhilodendron eziyivelakancane eziyivelakancane ngosiko lwezicubu.

Zibandakanye ocwaningweni nasekuhlanganyeleni nabanye abathanda izitshalo kanye nochwepheshe ukuze nishintshisane ngolwazi nemininingwane ngokutshalwa kwe-Philodendron ehlukahlukene esikweni lezicubu. Iya emihlanganweni yokucobelelana ngolwazi, izingqungquthela, kanye nemicimbi egxile ekusabalaliseni isiko lezicubu kanye nokuzalanisa izitshalo ukuze wandise amakhono akho nobungcweti ekuthuthukiseni ukutshalwa kwe-Philodendron ehlukahlukene. Ngokuhlala unolwazi futhi uxhumekile emphakathini wezitshalo, ungafunda mayelana nentuthuko entsha, izitayela, namathuba ekutshalweni kwe-Philodendron ehlukahlukene kanye nokusakazwa kwesiko lezicubu.

Yabelana ngolwazi lwakho nempumelelo yakho ekusakazeni izimila zePhilodendron eziyivelakancane ezingavamile ku-vitro nabanye abalimi nabaqoqi bezitshalo ukuze ukhuthaze futhi ufundise abanye abathanda. Bhala amasu akho okusakaza, imiphumela, kanye nokuphawulile ngendlela enemininingwane nephelele ukuze unikele olwazini oluhlangene lokutshalwa kwe-variegated Philodendron. Ngokwabelana ngothando lwakho lwezinhlobo zezitshalo zePhilodendron eziyivelakancane ezingavamile kanye nokusakazwa kwesiko lezicubu, ungasiza ukukhuthaza ukwazisa nokongiwa kwalezi zinhlobo zezitshalo eziyingqayizivele nezibalulekile phakathi kwabathandi bezitshalo emhlabeni wonke.

Isiphetho:

Sengiphetha, ukusabalalisa izinhlobo ze-Philodendron ezingavamile ze-variegated in vitro ngokusebenzisa amasu amasiko ezicubu kunikeza ithuba elijabulisayo nelijabulisayo kubathandi bezitshalo ukwandisa ukuqoqwa kwabo kwalezi zinhlobo eziyigugu. Ngokulandela izinyathelo ezivezwe kulo mhlahlandlela, ungakwazi ukuqalisa isiko lezicubu kusuka ezitshalweni, usungule imigomo ye-micropropagation, futhi uthuthukise ukutshalwa kwe-Philodendron ehlukahlukene ngokusebenzisa amasu nezinqubo ezintsha. Ukusabalalisa isiko lezicubu kunikeza indlela eqhubekayo nephumelelayo yokuphindaphinda izitshalo ze-Philodendron eziyivelakancane eziyivelakancane kuyilapho kulondolozwa izici zazo eziyingqayizivele kanye nobuqotho bezakhi zofuzo. Ngokutshala isikhathi nomzamo ekusakazweni kwesiko lezicubu, ungaba neqhaza ekongiweni nasekusakazeni ama-cultivar e-Philodendron ayivelakancane ayivelakancane ukuze izizukulwane ezizayo zizijabulele. Yamukela ubuciko nesayensi yokusabalalisa isiko lezicubu ukuze uvule amandla ezitshalo ze-Philodendron eziyivelakancane futhi uhlakulele iqoqo elihlukahlukene nelinempilo lalezi zinhlobo zezitshalo ezihlukile. Qala uhambo lwakho lwamasiko ezicubu namuhla futhi ubuke izinhlobo zakho eziyivelakancane zePhilodendron zichuma futhi zichuma endaweni elawulwayo yesiko lezicubu. Siyabonga ngokufunda lo mhlahlandlela wokusabalalisa izinhlobo zezitshalo ze-Philodendron ezingavamile ezingavamile, futhi kwangathi imizamo yakho yesiko lezicubu ingahlangatshezwana nempumelelo nenjabulo ekuhlakuleleni lezi zinhlobo zezitshalo ezinhle nezihehayo.