Ukuthuthukisa Izimo: Ukuhlakulela I-Philodendron Yezicubu Ezikhulayo Zezicubu

Isingeniso:

Ama-Philodendron ayizitshalo zasendlini ezidumile ezaziwa ngamahlamvu awo aluhlaza kanye nezidingo eziphansi zokunakekelwa. Ngamaqabunga awo akhangayo amise okwenhliziyo, engeza ukuthinta kobuhle bezindawo ezishisayo kunoma iyiphi indawo. Ngenkathi izindlela zendabuko zokusabalalisa ama-philodendron zibandakanya ukusebenzisa ukusika kwe-stem noma imbewu, isiko lezicubu lithola ukuthandwa njengendlela ephumelela kakhulu futhi esheshayo yokusakaza. Ukwenziwa kwezicubu kuhlanganisa ukutshala izitshalo endaweni yaselabhorethri eyinyumba, okuvumela ukukhiqizwa kwezitshalo eziningi ezifana nofuzo. Ukuze uhlakulele ngempumelelo i-philodendron yesiko lezicubu ezikhulayo, kubalulekile ukwandisa izimo ukukhuthaza ukukhula okunempilo. Kulesi sihloko, sizohlola izici ezibalulekile okufanele zicatshangelwe ekuthuthukiseni izimo zokutshala i-philodendron yesiko lezicubu.

Ukubaluleka Kwezindlela Eziyinyumba Kusiko Lwezicubu

I-sterility ibaluleke kakhulu uma kukhulunywa ngesiko lezicubu njengoba noma yikuphi ukungcoliswa kungaholela ekuhlulekeni kwalo lonke isiko. Ukungcoliswa kungenzeka ngezinhlamvu ezihamba emoyeni, amagciwane, noma isikhunta, ezingangena kalula emithonjeni yezakhi futhi zithiye ukukhula kwezitshalo. Ukuqinisekisa ukukhiqizwa okuphumelelayo kwe-tissue philodendron, kubalulekile ukugcina indawo eyinyumba kuyo yonke inqubo.

Enye yezindlela eziyinhloko zokuthola inzalo ukusebenzisa i-laminar flow hood. Lesi sisetshenziswa esikhethekile sihlinzeka ngokugeleza okuqhubekayo komoya ohlungiwe we-HEPA, ukuvimbela ukungcola okuphuma emoyeni ukuthi kungafinyeleli imikhumbi yamasiko nezicubu zezitshalo. Ukwengeza, wonke amathuluzi, iziqukathi, nemidiya esetshenziswa kuyo yonke le nqubo kumele kufakwe inzalo ngendlela efanele. I-Autoclaving, esebenzisa isitimu esinomfutho ophezulu, iyindlela ephumelela kakhulu yokuvala inzalo. Kufanele futhi kuqashelwe ukuhlanzeka komuntu siqu, okuhlanganisa ukugqoka amagilavu ​​kanye nokubulala amagciwane izandla njalo.

Ukukhetha Imithombo Yezokudla Efanelekile

Imithombo yezomsoco isebenza njengomthombo oyinhloko wezakhi ze-tissue culture philodendron, ehlinzeka ngazo zonke izakhi ezibalulekile ezidingekayo ekukhuleni. Kunezinhlobo eziningana zemidiya yezokudla ezitholakalayo ezithengiswayo eziye zabonakala ziyimpumelelo kusiko lwezicubu ze-philodendron. Phakathi kwazo, i-Murashige ne-Skoog (MS) medium isetshenziswa kakhulu futhi inikeza ukulingana okulinganayo kwama-macronutrients, ama-micronutrients, amavithamini, nezilawuli zokukhula kwezitshalo.

Kubalulekile ukukhetha indlela efanele yezakhi ezisekelwe ezidingweni ezithile zesiko lezicubu ze-philodendron. Ukuhlangana kwezilawuli zokukhula kwezitshalo, njengama-auxins nama-cytokinins, kungalungiswa ukuze kuthuthukiswe ukunwetshwa kwehlumela nokumila kwezimpande. Ukwengeza, ukungezwa kwamalahle acushiwe phakathi kungasiza ukususa imikhiqizo enobuthi futhi kuthuthukise ukukhula okuphelele kwe-tissue culture philodendron.

Indawo elawulwayo: Izinga lokushisa nokukhanya

Ukudala nokugcina indawo elawulwayo kubalulekile ukuze kutshalwe ngempumelelo i-tissue culture philodendron. Izinga lokushisa nokukhanya yizici ezimbili ezibalulekile ezithonya kakhulu ukukhula nokukhula kwezitshalo.

I-Philodendron tissue culture ivame ukuchuma ezingeni lokushisa elingu-25-30°C (77-86°F). Ukugcina izinga lokushisa elingaguquki phakathi kwalolu hlu kukhuthaza ukunwetshwa kwehlumela nokukhula kwezimpande. Ukushintshashintsha kwezinga lokushisa ngaphandle kwalolu hlu kungaba nomthelela omubi ekukhuleni kwesitshalo futhi kuholele kokungajwayelekile.

Izimo zokukhanyisa ezifanele nazo zibalulekile ku-tissue culture philodendron. Nakuba ama-philodendron engakwazi ukubekezelela ububanzi obuningi bokukhanya, ukunikeza ukukhanya okumaphakathi kuya phezulu kuyanconywa ukuze kukhule kahle. Ukukhanyisa okungeziwe okunemithombo yokwenziwa, njengezibani ze-fluorescent noma izibani ze-LED, kungasetshenziswa ukunikeza izimo zokukhanya ezingaguquki nezilawulwayo. I-photoperiod yamahora angu-12-16 ngokuvamile yanele i-tissue culture philodendron.

Ukungenisa umoya Okwanele kanye Nomswakama

Nakuba ukunakekela indawo eyinyumba kubalulekile, kubalulekile ngokufanayo ukunikeza umoya owanele ukuvimbela ukunqwabelana komswakamo owedlulele. Amazinga aphezulu omswakamo angakhuthaza ukukhula kwesikhunta namagciwane, okuholela ekungcoleni kanye nemiphumela elimazayo kusikompilo lwezicubu i-philodendron.

Ukungena komoya ngendlela efanele, kungaba ngokugeleza komoya kwemvelo noma izinhlelo zemishini, kusiza ukuvimbela ukwakheka kokujiya emithanjeni yesiko kanye nezicubu zezitshalo. Ukungenisa komoya kusiza futhi ekushintshisaneni kwegesi, okuqinisekisa ukutholakala okwanele kwe-carbon dioxide ye-photosynthesis. Kodwa-ke, kufanele kuqashelwe ukuthi akwethulwa ukungcola ngomoya ongenayo noma okusalungiswa.

Ukugcina izinga elifanele lomswakamo nakho kubalulekile ekutshaleni ngempumelelo isiko lezicubu ze-philodendron. Izinga lokuswakama lika-70-90% ngokuvamile liyanconywa ukuze kuncishiswe ukulahlekelwa kwamanzi ezicutshini zesitshalo futhi kukhuthaze ukukhula okunempilo. Lokhu kungafezwa ngokuvala iziqukathi ze-tissue culture endaweni elawulwayo enomswakama ophezulu noma ngokusebenzisa amagumbi okuswakama noma ama-misting systems.

Ukuqapha Okuvamile kanye Nezamasiko Ahlukene

Ukuqapha njalo amasiko e-tissue philodendron kubalulekile ukuze kutholwe noma yiziphi izimpawu zokuqala zokutheleleka noma ukukhula okungajwayelekile. Ukuhlolwa okubonakalayo kwamasiko kungasiza ekuboneni ukungcoliswa, ukuguquguquka kombala, noma i-tissue necrosis. Noma yimaphi amasiko angcolile noma angajwayelekile kufanele asuswe ngokushesha futhi alahlwe ukuze kuvinjelwe ukusakazeka okwengeziwe.

I-subculturing evamile nayo iyadingeka ukuvimbela ukuqoqwa kwemikhiqizo enobuthi nokugcina ukukhula namandla e-tissue culture philodendron. I-Subculturing ihlanganisa ukudlulisa ingxenye encane yesitshalo esikhula ngokukhuthele endaweni entsha yezakhi. Lokhu kuqinisekisa ukutholakala njalo kwezakhi kanye nezilawuli zokukhula, ukuvimbela ukuncipha kwezingxenye ezibalulekile ezidingekayo ekukhuleni okunempilo.

Isifinyezo

Ukuhlakulela i-philodendron yezicubu ezikhulayo kudinga inhlanganisela yezimo ezifanele nokuqapha ngokucophelela. Ukugcina inzalo kuyo yonke inqubo, ukukhetha indawo efanele yezakhi, ukuhlinzeka ngezimo zemvelo ezilawulwayo, nokuqapha njalo kanye ne-subculture kubalulekile ekukhuleni kwesiko lezicubu eziphumelelayo. Ngokunakekelwa okufanele nokunaka lezi zici, ungajabulela izinzuzo zokusakazeka ngokushesha futhi ukhiqize izitshalo ze-philodendron ezinempilo nezinempilo ngokusebenzisa isiko lezicubu.